BACILLUS COLI AND SALTS OF BILE 51 
Broth 
Milk 
Glucose Jelly 
I. 
Motile. Short bac. . 
General 
turbidity 
Acid and 
clotting 
Gas 
No liquefaction 
Moist, grey 
yellow. P.D. 
+ 
» 
» 
» 
V 
+ 
3- 
Slightly motile. Short bac. 
» 
» 
Almost invisible 
+ 
4- 
Actively „ „ 
" 
» 
» 
Moist,yellow. P.D. 
+ 
5- 
" " " 
+ 
6. 
? Motile 
+ 
7- 
Actively motile „ 
" " 
+ 
8. 
Slightly 
» 
» 
Moist, grey 
yellow. P.D. 
+ 
9- 
Motile „ 
„ 
Moist, grey. P.D. 
+ 
10. 
Actively motile „ 
Almost invisible 
Motile „ 
Moist, yellowish. 
P.D. 
+ 
P.D.= discolouration of potato. 
Again, 5 c.c. of the Ashton Hall Septic Tank liquor were mixed with 95 c.c. 
of the earth, road sweepings, and tap water mixture used in the previous experiment, 
and which had been standing on the laboratory bench. 1 c.c. of the mixture was put 
into each plate, and the medium used was B. s. 0.5 p.c, peptone 2 p.c, lactose 1 p.c, 
agar 1.5 p.c, tap water. The average of three plates was 11 94 colonies per plate. 
The whole of the plate was ' milky,' except for a small clear space round some of the 
colonies. The colonies with a clear space round them appeared to be of two kinds. 
One was finely and uniformly granular, with a regular, well-defined margin ; the other 
had an irregular edge, and was covered with fine wavy lines. Cultures in broth were 
made from eight colonies. 
Broth 
Milk 
Glucose Jelly 
Potato 
Indol 
1. Granular colony 
General 
Acid and 
Gas 
Moist, grey 
+ 
with clear space. 
Short B., motile 
turbidity 
clotting 
No liquefaction 
P.D. 
„ ? motile 
+ 
3- 
,, ? motile 
+ 
4- 
,, motile 
+ 
5. Hazy colony . 
+ 
6. „ 
„ actively motile 
+ 
7. Lined „ 
" 
+ 
8. „ 
,, motile 
+ 
