BACILLUS COLI AND SALTS OF BILE 53 
III. There were 122 colonies on this plate. It was exposed to light for 
several days. Three of the surface colonies turned yellow ; the rest remained 
greyish and translucent. The 3 yellow colonies and 3 others were transferred to broth. 
Colony 
Organism 
Broth 
Milk 
Glucose Jelly 
Potato 
Indol 
Yellow . . 
Short bac, actively motile 
General 
Clotting 
No liquefaction 
Moist, 
turbidity 
and acid 
Gas 
yellow. P.D. 
2. 
+ 
3- 
+ 
4- 
Grey . . . 
„ motile 
Slight acid 
No liquefaction 
Just 
+ 
No clotting 
No gas 
visible 
5- 
+ 
6. 
+ 
The mixture of earth, &c, remained in the cupboard for five more days, when 
it was again plated on nutrient agar and on lactose bile salt agar. On the nutrient 
agar plate there were about 70 colonies of various kinds, of which five or six might 
have been B. c. c. or B. t. a. 
On the bile salt agar plate there were four colonies. Cultures were made from 
two of them. Both gave cultural characteristics corresponding to the B. t. a. One 
was tested with serum and gave a positive reaction ; it also gave the indol reaction. 
Five days later this mixture was again plated in bile salt lactose agar, 1 c.c. 
being put into the plate. Only one colony, besides a few moulds, appeared, and this 
was B. c. c. 
One c.c. of urine, from a case* of enteric fever, was put into a plate of bile salt 
lactose agar. The result was an almost pure culture of B. c. c. A few of the colonies 
seemed as if they might be B. t. a., and these were transferred to broth and subcultured. 
Broth 
Milk 
Glucose Jelly 
Agar 
Indol 
I. 
Slender bac 
Extremely motile 
General 
turbidity 
Greenish yellow 
fluorescence 
Acid clotting 
Peptonization 
Greenish yellow 
liquid 
No Gas 
liquefaction 
Greenish yellow 
colour 
Bac. Pyocyaneus 
+ 
2. 
„ „ 
» 
+ 
3- 
+ 
4- 
Short bac. 
Slightly motile . 
General 
turbidity 
Acid and 
clotting 
No liquefaction 
Gas 
Dry, filmy, greyish j No spores, 
translucent > Not by 
growth j Gram. 
+ 
5- 
+ 
* This case was a typical case subsiding by lysis. 
The specimen was taken on the 24th day. The temperature was normal. The serum reaction had been tried 3 times, 
with 2 negative results and 1 doubtful. 
