BACILLUS COT J AND SALTS OF BILE 55 
The following samples were plated in Bile salt 0.5 p.c., peptone 2 p.c, 
lactose 1 p.c., agar 1.5 p.c. : — 
I. Road puddle diluted with sterile water. Carbol agar (Miss Chick's analysis). 
1. 200 B. c. c. "I 
2. 212 „ > = 195 . . Average 211 
3- » * 
II. Road dust, shaken up with sterile water. 
1. 13 B. c. c. ^ 
2. 11 „ =11 . . Average 3 
3- 9 ,1 ' 
III. Earth from a potato field, shaken up with sterile water. 
l ' 1 
2. J- 0 B. c. c. . . . . Average o 
3- J 
IV. Earth from another potato field, shaken up with sterile water. 
2. 1 Sterile, except for a few moulds . Average o 
3- J 
V. Garden earth, in which turnips were growing, shaken up with sterile water. 
1. 1 B. c. c. 1 
2. 1 „ > = 1 . . . Average 3 
3-i » ' 
Samples I, II, and III contained, besides B. c. c, another kind of colony which 
was very small, round, grey, slightly raised, and looked finely granular under the 
microscope. Eleven cultivations were made from these colonies, and all turned out 
to be yoasts. 
Plates were also made with water from the aqueducts at Vyrnwy and 
Rivington. Three plates were made from each sample, 1 c.c. of water being put into 
each plate. 
There was no growth on any of these plates. 
A sample of water from a pond, in which there were 20,000 young trout, was 
plated in the same medium, 1 c.c. being put into each of 3 plates. There was no 
growth on any of the plates. 
Countings were made of 13 samples of Severn water, and it was found that 
when there were not many colonies on a plate the number agreed with those on carbol 
agar ; but when the colonies were more than about 100 per plate other organisms 
interfered with the proper development of the B. c. c. 
