BACILLUS COLI AND SALTS OF BILE 57 
These experiments are so few that a definite conclusion cannot be framed, but 
they suggest that working on these lines it may be ultimately possible to devise a 
simple method of isolating the Bacillus typhi abdominalis. 
In making up the medium great care must be taken not to overheat it after 
adding the lactose, as, in that case, a change takes place, and B. t. a. produces a haze 
like B. c. c. The composition which has given the best results so far is : — Bile salt 
0.5 p.c, peptone 2.0 p.c, lactose 1.0 p.c, agar 1.5 p.c, tap-water q. s. 
H 
