DISTRIBUTION OF BACILLUS ENTERITIDIS SPOROGENES 
If the culture is less virulent the post-mortem appearances will be modified, 
the separation of skin will be over a less extensive area, and if the culture is very little 
pathogenic there may only be a slight separation of skin with only a small quantity of 
fluid in the space. In these cases the fluid is gradually absorbed and the separated 
skin dries, and after one to two weeks sloughs off, leaving an ulcer. In a few cases 
the whey is quite non-pathogenic ; it is absorbed, and the animal shows no signs of 
being ill. 
1. Dried Salted Codfish. — A litmus milk tube was inoculated with a small shred ; after 
48 hours' incubation it had 'gone' typically. 2 c.c. whey were inoculated into a Guinea-pig. 
Result — Died in 18 hours. 
2. Dried Salted Haddock. -A litmus milk tube was inoculated with a small shred; after 
18 hours it had 'gone' typically. 2 c.c. whey were inoculated into a Guinea-pig. Result — It 
was found dead in 16 hours, the skin over the abdomen and thorax was completely separated 
from the subjacent muscular tissue and there was a considerable quantity of badly-smelling 
sanguineous fluid in the space. 
3. Boiled Cod and Potatoes. — Litmus milk tubes were inoculated with small portions of the 
fish and of the potato with negative results for B. enteritidis sporogenes. 
4. Salted Ling. — A litmus milk tube was inoculated with negative results for B. enteritidis 
sporogenes. 
5. Salted Cod. — A litmus milk tube was inoculated with a small shred ; after 18 hours' 
incubation it had ' gone,' but the whey was slightly yellow and had a putrid smell, a subculture 
of this in litmus milk 'went' and smelt of butyric acid. A Guinea-pig was inoculated with 
1 c.c. of the whey from the first tube. Result — Non-pathogenic. 
6. Salted Haddock. — A litmus milk tube was inoculated with a small shred with negative 
result for B. enteritidis sporogenes. 
7. Salted Cod. — A litmus milk tube was inoculated with a small shred with negative 
result for B. enteritidis sporogenes. 
8. Salted Cod. — A litmus milk tube was inoculated with a small shred from a clean part 
of the fish with negative results for B. enteritidis sporogenes. Another tube was inoculated with 
a shred from a spot where there was a mark as if from a dirty finger ; after 18 hours' incubation 
this tube had ' gone ' typically. A Guinea-pig was inoculated with 1 c.c. of the whey. Result — 
Died in 30 hours and had separation of the skin and red fluid in the space. 
9. Salted Cod. — A litmus milk tube was inoculated with a small shred. There was little 
change after 18 hours' incubation, but after some days it had 'gone.' l\ c.c. whey were 
inoculated into a Guinea-pig. Result — Died in about 40 hours with complete separation of skin 
over thorax and abdomen. 
10. Salted Cod. — A litmus milk tube was inoculated with a small shred; after 18 hours 
incubation it had 'gone' atypically and had a putrid smell. A Guinea-pig was inoculated with 
2 c.c. whey. Result — Died in 48 hours with considerable separation of skin and sloughing of 
abdominal muscle. 
11. Salted Cod. — A litmus milk tube was inoculated with a small shred with negative- 
results for B. enteritidis sporogenes. 
12. Salted Haddock. — A litmus milk tube was inoculated with a small shred ; after 18 
hours' incubation it had ' gone ' typically and well, the cream being scattered. A Guinea-pig way 
inoculated with 2 c.c. Result — -Pathogenic but not fatal. 
13. Salted Cod. — A litmus milk tube was inoculated with a small shred with negative 
results for B. enteritidis sporogenes. 
