DISTRIBUTION OF BACTERIUM COLI COMMUNE 3 
through a second culture in broth carbolized in the same proportion, a pure culture 
was obtained by stroking on agar ; in some cases, however, the culture so obtained 
was obviously mixed. Table I gives the details of the former cases — 10 samples of 
crude sewage from West Derby, Chorley, and Dewsbury, yielded pure cultures, all 
of which were liquefying organisms which also coagulated milk, and 4 of which 
were spore-bearing. Out of 29 cases of sewage effluents examined, 9 only gave a 
pure culture of B. coli commune, while 9 gave a liquefying organism, and in 1 1 
cases no growth at all was obtained. 
TABLE I 
Description of sample 
N 
a. of samples 
examined 
No. surviving 
1st incubation, 
24hrs.at42°C. 
1 c.c. in 50C.C 
containing 
39 c.c. sterile 
water and 
10 c.c. broth car- 
bolized in the 
proportion of 
5 in 1,000 
No. surviving 
2nd incubation, 
24 hrs. at 42° C. 
1 drop of latter 
added to 10 c.c. 
broth carbolized 
in the pro- 
portion of 
1 in 1,000 
No. giving 
liquefying 
organism, 
which also 
coagulated 
milk, but gave 
no indol or gas 
in glucose 
gelatine 
No. giving a 
spore-bearing 
liquefying 
organism 
No. giving an 
organism which 
agreed with 
B. coli com- 
mune, showing 
coagulation of 
milk, indol, gas 
in glucose 
gelatine, and 
no liquefaction, 
motile bacillus 
West Derby Sewage. . . 
Dewsbury Sewage ... 
Chorley Sewage 
5 
3 
2 
5 
3 
2 
5 
3 
2 
5 
3 
2 
2 
2 
Total Sewages... 
10 
10 
10 
10 
4 
Leeds Dibdin(No. 4.) 
Bed Effluent ... 
Leeds Lime Bed 
Effluent 
Leeds Cameron Bed 
Effluent 
West Derby Farm 
Land Effluent ... 
Dewsbury Effluent ... 
Chorley Tank Efflu- 
ent 
Chorley Filter Efflu- 
ent 
Ashton Hall (L'pool) 
(Experimental) 1st 
Effluent 
(Experimental) 2nd 
Effluent 
I 
2 
I/ 
2 
I 
2 
I 
2 
7 
2 
1 
2 
1 
1 
2 
6 
2 
2 
2 
4 
2 
2 
2 
I 
Total Sewage 
Effluents 
29 
19 
18 
9 
9 
Total 
39 
29 
2S 
'9 
4 
9 
