BACILLUS ENTERITIDIS OF KLEIN 137 
the site of inoculation, the term pathogenic is used to distinguish from the transient 
swelling resulting from inoculation with the non-pathogenic bacillus of Botkin. 
In all, except possibly two or three of the earlier experiments, the milk 
inoculated had been recently sterilised by not more, usually much less, than, five days 
previously ; a necessary precaution, because stale milk becomes again charged with 
air (oxygen) by absorption, and is consequently a bad anaerobic medium. 
Cases 
Enteritidis Spores 
Dilution 
Nature of Stool 
Result of Inoculation 
I to 9 
Absent 
260 
IO 
Absent 
+ 
1 I 
Absent 
2 
I 2 
Present 
I 3OO 
Fluid 
Diminished virulence 
13 
Present 
I 3OO 
Solid 
Pathogenic 
Botkin ? 
I 30O 
Semi -sol id 
Non-pathogenic 
!5 
Present 
260 
Semi -sol id 
Diminished virulence 
16 
Present 
I 3OO 
Fluid 
Normal virulence 
'7 
Present 
65 
Fluid 
Pathogenic 
Cases 1 to 1 1 were suffering from the following diseases amongst others — 
rheumatism, morbus cordis, phthisis, acute bronchitis, and pneumonia. 
Cases 14 and 1 5 had cirrhosis of liver, and 17 enteritis. 
In cases 1 to 1 1 enteritidis spores were only proved to be absent in very small 
portions of stool, while they were occasionally present in still smaller portions ; when 
the number per gramme must have been enormous. If comparatively large quantities 
of stool, say from one to three loopfuls, had been tested, it is highly probable the 
spores would have been isolated in almost every instance. 
Having therefore, contrary to expectation, found enteritidis spores in the 
diarrhoeal evacuations of patients, only one of whom had symptoms of intestinal 
irritation, several normal evacuations were next examined, but the amount tested 
was larger than the first series. Samples 1 to 5 were obtained from hospital patients 
whose bowels were moved about once a day ; number 6 was a healthy individual. 
