THOMPSON YATES LABORATORIES REPORT 
Cases 
Enteritidis Spores 
Dilution 

Nature of Stool 

Result of Inoculation 
I 

Present 
150 
Relaxed 
Diminished virulence 
2 
Botkin r 
2 
Relaxed 
Non-pathogenic 
3 
Present 
I 50 
Normal 
Normal virulence 
+ 
Present 
Small particle 
Normal 
Diminished virulence 
5 
Present 
Small particle 
Normal 
Normal virulence 
6 
Present 
2 
Normal 
Pathogenic 
Thus the spores were present in five out of six samples of relaxed or normal 
stools obtained from different individuals, while they were absent in a large 
percentage of the diarrhoeal cases, because, I believe, a relatively smaller quantity 
was tested. 
But assuming that the same number of enteritidis spores were present in an 
equal quantity of faecal material from two persons, one only suffering from 
diarrhoea, it is incorrect to infer that, therefore, enteritidis is sporing with equal 
rapidity, and that the spores are equally numerous in the intestines of each ; because 
the total quantity of diarrhoeal stool passed in a given time will, in most instances, 
greatly exceed the total quantity of normal stools. 
The former is diluted with fluid, and consequently its bulk is increased, so 
the organisms present are also diluted. In other words, one loopful of a diarrhoeal 
evacuation represents a much smaller portion of the whole quantity passed in twenty- 
four hours than one loopful of the normal evacuation. In order, therefore, to compare 
accurately the number of enteritidis spores present in the intestines of two persons 
passing normal and diarrhoeal motions, respectively, it would be necessary to collect 
and measure the total quantity passed in twenty-four hours, minus urine ; then to 
ascertain the smallest measured portion in which the spores were present, and from 
that to calculate the total number of spores passed per diem : a tedious proceeding not 
devoid of practical difficulties. 
Now the total quantity of diarrhoeal stool passed in twenty-four hours exceeds 
that of the normal, partly because it is diluted with water which would tend to lessen 
the specific gravity. I therefore thought that if diarrhoeal and normal evacuations were 
made into an emulsion with sterile water and diluted to a standard specific gravity, 
the bulk of the former would be slightly increased and the bulk of the latter greatly 
increased ; and then if the same quantity of both emulsions were examined for the 
spores of enteritidis, a rough comparison of the total number in both kinds of stool 
might be obtained without collecting and measuring the whole quantity passed in a 
day. But I have found the method unsatisfactory, because the specific gravity of a 
diarrhoeal evacuation does not always vary with its consistency. 
