BACILLUS ENTERITIDIS OF KLEIN 139 
Below are appended the results of the examination of fourteen consecutive 
samples of faecal material, the first series were from patients suffering from diarrhoea, 
the remainder from perfectly healthy individuals who were not in hospital. Each 
sample was made into an emulsion with sterile water and reduced to a specific gravity 
of 1005. Next, one cubic centimetre of the emulsion having been previously well 
stirred, was inoculated into a freshly sterilised tube of litmus milk. Some of the 
emulsion was also diluted 100, 10,000, and 1,000,000 times, and one cubic 
centimetre of each dilution was also inoculated into milk. The four milk tubes 
from each sample were then heated, incubated anaerobically, and at the end of thirty- 
six to forty-eight hours the whey of a typical milk was inoculated into a guinea-pig 
in the proportion of one cubic centimetre to every 200 grammes. 
DIARRHOEA 
Cases 
Spores in 
Dilution of 
Result of Inoculation 
1 
10,000 
1,000,000 
I 
Present 
Present 
Pathogenic 
2 
Present 
Present 
Normal virulence 
3 
Atypical 
Not inoculated 
4 
Botkin (?) 
Botkin (?) 
Non-pathogenic 
5 
Present 
Present 
Normal virulence 
6 
Present 
Present 
Present 
Normal virulence 
7 
Present 
Normal virulence 
Case 1 had ovarian cyst, 2 pneumonia, 6 tubercular enteritis, and 7 paren- 
chymatous nephritis. 
NORMAL 
Cases 
Spores in 
Dilution of 
Result of Inoculation 
1 
100 
10,000 
1,000,000 
I 
Present 
Normal virulence 
2 
Present 
Present 
Pathogenic 
3 
Present 
Normal virulence 
+ 
Present 
Present 
Present 
Pathogenic 
5 
Present 
Present 
Pathogenic 
6 
Botkin (?) 
Botkin (?) 
Non-pathogenic 
7 
Present 
Present 
Pathogenic 
