152 
THOMPSON YATES LABORATORIES REPORT 
the centre. A few have a haze round them. The 
size is about twice that of B. typhi abdominalis. 
Deep colonies— Lens-shaped, orange white, and all have a haze round 
them. 
This haze makes the colonies appear larger than they 
really are. A drop or two of a solution of ammonia 
placed upon the colony will cause the haze to disappear. 
If the plates be exposed to the light for several days 
it will be found that the colour of the colonies has 
deepened and that those on the surface have a ten- 
dency to become mucoid. 
B. Enteritidis (G /T,rtnkr) 
B. of Hog Cholera (Flexner) 
B. of Psittacosis (Nocard) 
B. Pyogenes foetidus 
B. Neapolitanus 
B. fluorescens liquefaciens 
Cocci. 
| Colonies similar to those of B. typhi 
( abdominalis 
~| Colonies similar to those of B. coli 
I communis. 
| Slightly raised, flattened, roundish, 
translucent, greenish-white colon- 
) ies with very slight fluorescence. 
Surface colonies — Round, raised, opaque-white. 
Deep colonies — Usually opaque white. Under the microscope they 
appear black with a rough irregular hairy edge. 
The following few organisms have been tried and apparently do not grow on 
this medium at 42 0 C. 
B. Megatherium. 
B. Subtilis. 
B. Filamentosus. 
Sarcina lutea. 
Torula alba. 
The following experiment was performed to ascertain the inhibitory power at 
42 0 C. of this medium upon ordinary earth organisms. 
About two to three grammes of garden mould were shaken up with 100 c.c. of 
tap water and 1 c.c. of the mixture put into a tube and poured into a plate. 
At the end of forty-eight hours there were, besides many moulds, ten 
colonies (both surface and deep). All these colonies were subcultured, and nine gave 
a growth. These nine were then cultured in different media and the reaction noted. 
The incubation temperature was always 42 0 C. 
The following table gives the reactions of these nine organisms : 
