238 THOMPSON YATES LABORATORIES REPORT 
Experiment III — A number of male and female Anopheles were introduced into 
a cage with water and banana. The females were all fed on blood once only, before 
introduction ; all died within four days — no ova in water or in insect. 
Experiment IV — Repeated this experiment with a large number of males and 
females. The females fed once on blood, afterwards on banana and water. Some 
few lived twelve days, all were dead on fourteenth ; no ova found. Ovaries unde- 
veloped ; spermathecae empty. 
Experiment V- — Introduced into a cage a number of females and males, the 
females had not been in contact with males before feeding : they were fed only once 
on blood. Five days afterwards, no eggs having been laid, a further number of males 
and females were introduced, the females having been in contact with males since 
birth — and having had one feed of blood. Banana and water were kept freshly 
supplied daily in the cage. All died within eighteen days from the first, thirteen days 
from the second introduction ; no eggs having been laid. 
Experiment VI — A number of Cu/ex males and females were placed in a cage 
with banana and water ; they had no blood ; but lived for sixteen days — laying no 
eggs. 
Experiment VII — A number of Cu/ex males and females introduced with banana 
and water. The females were fed on blood on introduction and again seven days 
afterwards. Eggs were laid on the ninth day of the experiment, two days after the 
last feed of blood. 
These experiments were performed in small cages, made of mosquito netting, 
of size about twelve inches long, twelve inches deep, and six inches wide. 
Among mosquitoes of the genus Cu/ex, copulation was often observed in the 
small cages of our experiments, but with Anopheles never. It was thought possible 
that these latter required a longer flight than could be obtained in cages of the 
dimensions given, so that our next experiments were made with a large cage eight 
feet long by six deep by four wide, into which were introduced a small iron tank and 
a soil puddle in an earthenware vessel containing water and a number of peeled 
bananas which were daily replenished. 
Experiment VIII 
June 8 Thirteen newly-hatched males and twelve newly-hatched 
females (Anopheles) were introduced into the large cage, 
the females have been fed on the blood of one of us. 
,, 9 Introduced some males caught in native quarters and four 
newly-hatched, blood-fed females. 
,, 10 Introduced eight blood-fed females and a number of males, 
all newly-hatched out. 
„ 13 Re-fed thirteen of the above females, some would not feed, 
others had died. 
