MALARIA EXPEDITION TO NIGERIA 
239 
June 15 Re-fed nine of the above females; introduced ten more 
freshly-hatched, blood-fed females and ten males, three of 
which were caught in the native town ; three females were 
found dead on the water. 
„ 17 Re-fed thirteen females; one found dead on the water; 
showed undeveloped ovaries and empty spermatheca. 
,, 19 Re-fed eleven females ; introduced three caught males from the 
native town. 
„ 21 Re-fed ten females ; only one living male can be seen. First 
noticed the ovaries beginning to swell as a greyish patch on 
upper surface of abdomen. 
„ 23 Re-fed eight females. 
„ 24 Re-fed six females ; no males can be seen. 
„ 26 Re-fed eight females ; one female was accidentally killed while 
feeding. Ova were found fully developed enclosed in their 
usual chitinous ridged capsule. No spermatozoa in the 
spermatheca. 
„ 28 Re-fed nine females — all except one shewed distended abdomens 
„ 30 Introduced three females caught in native town. Re-fed nine 
females. 
July 2 Re-fed eight females. 
„ 5 Re-fed six females ; eggs were found on the surface of the 
water in the small iron tank. All the females were apparently 
full of eggs except one, presumably the one which had laid 
the eggs. This mosquito was caught and kept by herself 
in a small cage. 
7 Only four females remain in I The isolated female was re-fed on blood, 
the large cage, two of which 
would not feed — also two 
males present. 
9 Re-fed four females in large 
cage. 
1 1 Re-fed four females. 
The four females and two 
males remaining in the 
large cage were removed 
and placed in a small cage. 
This female laid more eggs ; she was 
again re- fed. The ova of the 5th 
had not hatched out. 
More eggs laid ; re-fed with blood ; 
eggs of 5th and 7th had not 
hatched out. 
Few more eggs laid. None of the pre- 
vious eg-p-s hatched. 
