THOMPSON YATES LABORATORIES REPORT 
July 19 Re-fed the females. 
22 
2 5 
Re-fed the females. 
Five females re-fed. 
„ 27 Re-fed two females. 
„ 29 Re-fed two females ; ovaries beginning to swell. 
„ 3 1 Re-fed the two females. 
Aug. 2 Re-fed the two females ; swelling of abdomen more pronounced 
,, 4 Again re-fed. 
„ 7 Re-fed. 
„ 9 Re-fed. 
,, 1 1 Would not feed. 
,, 13 One had disappeared ; the other would not feed. 
,, 1 5 Re-fed both females ; abdomen very swollen ; no eggs laid. 
,, 19 Both had disappeared. (Probably eaten by ants). 
In order to show that blood is necessary for the development of the ovaries and the 
formation of developed ova, the following experiment was performed. The females 
were not fed on blood. 
Experiment XI 
July 8 Ten females introduced into a small cage with a number of 
males caught in the native town ; no blood was given ; 
banana and water also placed in cage. 
,, 16 Ten females still alive ; the banana was replenished every day. 
,, 1 8 Eight females alive ; no eggs ; one dead female examined 
showed undeveloped ovaries. 
„ 2 1 More males caught in native town introduced. 
,, 25 One female dead on the water; showed undeveloped ovaries. 
„ 27 Only one female left ; no eggs laid. 
„ 29 All dead ; no eggs laid. 
Thus, without feeding on blood, female Anopheles may live at least twenty-two days 
on vegetable juices alone, but the ovaries remain undeveloped, although many males 
may be present. 
The next experiment indicates that a regular and frequent blood feed is 
necessary — as often as every other day, every fourth day being insufficient — for the 
development of ova. 
Experiment XII 
July 1 8 Ten female Anopheles, bred from pupae, introduced into a 
small cage with six males caught in native town. 
,, 20 Two females had died. 
