THE BACTERIOLOGY OF THE OYSTER AM) uTIIER SHELLFISH 
45 
EXI'ERIMEXT VI. 
At time of luixin- ... ... ... ... ... ... 325 
After 172 hours ... ... ... ... ... ... 2 
Experiment VII. 
At time of mi.xiny ... ... ... ... ... ... 325 
After 504 hours (water kept at 8 C— 10' C.) ... ... ... 79 
KXI'ERIMENT VIII. 
At time of mixing ... ... ... ... ... ... 325 
After 504 hours ... ... ... ... ... ... o 
III. — The InfectioiX of the Oyster with the 1>. tyfiiosus, and its 
Removal by Washing. 
These expenment,s were undertaken to ascertain whether oysters offered a favour- 
able nidus for the multiplication of typhoid bacilli. 
Healthy oysters were placed in large glass vessels containing sea-water, to which 
was added surface scrapings from agar plates previously inoculated with the typhoid 
bacillus. 
By means of a syringe the water was aerated once or twice daily. 
The contents of the stomach were pipetted out in the manner that will be 
indicated further on, and agar plates made ; at the same time the water in which the 
oyster was placed was likewise analysed. 
Table slioiving number of Organisms present in Stomacli after infecti]ig nutter. 
Oyster. 
Inoculated. 
Examined. 
No. of 
Colonies. 
Agar. 
Organisms 
present 
in Oysters. 
Number present in the 
Sea-water. 
August 25. 
August 26. 
1,700 
Ahnost entirely 
Water in the same case 
typhoid. 
585,000 per c.c- 
3 
August 27. 
7,020 
,, 
Water in the same case 
468,000 per c.c. 
4 
August 28. 
7,000 
Water in same case 
40,950 on agar, 
5,200 on gelatine. 
5 
August 26. 
August 29. 
453 
6 
August 28. 
August 30. 
195 
Water in same case 
7 
September 4. 
390 
2,047,500 per c.c. 
8 
August 31. 
3-5 
September 10. 
455 
The above table shows that the typhoid bacillus does not increase in the body or in 
the tissues of the oyster. The figures would rather indicate, comparing the large number 
of bacilli present in the water with those found in the alimentary tract, that the bacilli 
perish in the intestine. We showed their presence up to ten days, and Dr. Klein up to two 
or three weeks. 
