46 
UYSTERS AND DISEASE. 
In the next scries of experiments infected oysters were taken, the duplicates of 
which, as seen in the above table, contained comparatively large numbers of the B. typhosus. 
They were subjected to a running stream of pure clean sea-water. The result was definite 
and uniform ; there was a great diminution or total disappearance of the B. typhosus in 
from one to seven days. 
Uyster. 
Inoculated. 
Wabhed. 
E.xamined. 
No. of 
Colonies. 
Agar. 
Kind of Organisms present. 
I 
August 25. 
August 26. 
August 30. 
80 
2 Colonies, B. typhosus. 
2 
August 28. 
23 
B. typhosus present. 
3 
August 26. 
,, 
44 
4 
August 29. 
40 
5 
August 27. 
„ 
5 
6 
„ 
August 31. 
700 
Abundant B. typhosus. 
7 
August 28. 
.August 30. 
55 
B. typhosus present. 
8 
August 26. 
August 28. 
September 3. 
4 
? B. typhosus. 
9 
August 27. 
August 29. 
10 
No B. typhosus found. 
lO 
8 
3 Colonies of B. typhosus. 
1 1 
August 28. 
August 30. 
September 4. 
4 
I Colony of B. typhosus. 
12 
September 3. 
200 
Majority B. typhosus. 
13 
August 31. 
? 
4 
>4 
.August 28. 
September 3. 
September 6. 
No B. typhosus, but Proteus. 
15 
August 31. 
5 
? B, typhosus. 
i6 
September 5. 
70 
One-half Colonies B. typhosus. 
17 
September 3. 
September 10. 
No B. typhosus. 
i8 
September 5. 
September 11. 
? B. typhosus. 
IV. —The Bacteria present in the Alimentary Canal of the Oyster and 
THE Mussel and other Shellfish. 
The method of analysis consisted in first cauterizing the mantle over the region of 
the stomach and then inserting a fine sterilized glass pipette ; the pipette was moved 
about, and when sufficient of the contents of the stomach and the juices of the oyster 
had risen in the pipette, the latter was removed and its contents transferred to liquefied 
agar, ordinary gelatine, or sea-water gelatine, and plate cultivations made. In the first 
six cases examined {see Table, A— F) the oysters were especially fresh ; in all the other 
cases the shellfish had been obtained hap-hazard from the various shops. In a very 
large proportion of cases to yC) the B. colt was isolated. In very many instances 
this organism was present in overwhelming numbers in the Petri dish. 
