8 THOMPSON YATES AND JOHNSTON LABORATORIES REPORT 
Experimental Inoculations 
The following animals were inoculated from this case : — 
Animals Inoculated from Case 4 
September 30. White rat (Experiment 5) inoculated subcutaneously with 
5 c.cm. blood ; never infected. 
September 30. White rat (Experiment 4) inoculated subcutaneously with 
1 c.cm. blood ; infected October 8. 
October 27. Guinea-pig (Experiment 27) inoculated subcutaneously with 
4 c.cm. blood, infected November 7. 
October 27. Guinea-pig (Experiment 28) inoculated subcutaneously with 
5 c.cm. blood ; infected November 18. 
October 27. Rabbit (Experiment 24) inoculated subcutaneously with 6 c.cm. 
blood ; never infected. 
Necropsies were done at Boma on four cases which were admitted to the hospital 
for sleeping sickness. Two of these died before their blood or cerebro-spinal fluid 
could be examined. Repeated examinations of the blood (centrifuge used) of 
the third failed to demonstrate trypanosomes. Lumbar puncture was not done 
on this case. In spite of repeated and very careful examinations of the blood and 
cerebro-spinal fluid of the fourth case, trypanosomes were never seen. 
A necropsy was also done on the body of a native admitted to the hospital as a 
case of encephalitis. This patient never showed the usual signs of sleeping sickness, 
and Anally died of dysentry. Many trypanosomes were seen in his finger blood. 
Lumbar puncture was not done. 
The post-mortem appearances in each of these cases were very similar to those 
described as occurring in sleeping sickness. 3 The usual increase of subarachnoid 
fluid, which had become cloudy, or occasionally almost purulent, was observed. The 
superficial and substantial vessels of the brain and spinal cord were turgid, and in two 
cases small sub-ependymal haemorrhages were noted. 
In addition to these changes in each case, lymphatic glands were enlarged, several 
were congested or injected, not infrequently members of the various groups of glands 
were to the eye either partially or — especially the smallest glands — totally haemorr- 
hagic. The naked-eye appearances of these glands were particularly interesting to 
us since we have observed very similar changes in animals infected by us with 
Trypanosoma gambiense. 
Experimental Inoculations 
The following experimental inoculations were made in white rats, white mice, 
rabbits, and guinea-pigs, with the results indicated : — 
Twenty white rats were inoculated with cerebro-spinal fluid or blood taken 
