75 
A COMPARISON OF THE ANIMAL REACTIONS OF THE 
TRYPANOSOMES OF UGANDA AND CONGO FREE 
STATE SLEEPING SICKNESS WITH THOSE OF 
TRYPANOSOMA GAMBIENSE* 
(dutton) 
A PRELIMINARY REPORT' 
BY 
H. WOLFERSTAN THOMAS, M.D., CM., McGill 
J. H. TODD MEMORIAL FELLOW IN TROPICAL MEDICINE 
AND 
STANLEY F. LINTON, M.B., B.Sc, Vict. 
ASSISTANT DEMONSTRATOR 
{From the Johnston Tropical Laboratory, University of Liverpool) 
THE strains we have used in our work are as follows : — Three strains of 
Trypanosoma gambiense from Gambia. (See below). Two strains from 
Uganda : (i) from the cerebro-spinal fluid of a case of sleeping-sickness ; 
and (2) from the blood of a case of trypanosome fever. We take this opportunity 
of thanking Colonel D. Bruce, R.A.M.C., F.R.S., to whom we are indebted for 
these two strains. Four strains from the Congo Free State : (i) 1 from the cerebro- 
spinal fluid of a case of sleeping sickness ; (2) 1 from the blood of a case of trypanosome 
fever ; and (3) and (4) from the blood of two natives at present under observation 
in Liverpool. 
Trypanosoma gambiense (Dutton). — Three strains of Trypanosoma gambiense were 
brought home by Dr. J. E. Dutton and Dr. J. L. Todd, from their Senegambia 
expedition' ; of these three, which are called ' Gunjur,' ' Lamm in,' and 'Q' strains, 
we have worked almost entirely with the 'Gunjur' strain, which was derived from 
the boy at Gunjur, case 4. 4 Since that time the strain has been passed through a 
great number of animals under varying conditions ; the direct strain is that passed 
from rat to rat, to ascertain whether increased virulence or attenuation of the try- 
panosomes could be produced. It is not our purpose to present here the results, but 
* Reprinted by permission of the publishers of the Lancet, l^th May, 1904 
1. In the forthcoming Thompson Yates and Johnston Laboratories Reports a fuller report will be published, together 
with serum and agglutination experiments, and the results of therapeutic experiments. 
2. Sent to us by our expedition to the Congo Free State. 
3. J. E. Dutton and J. L. Todd, First Report of the Trypanosomiasis Expedition to Senegambia (1902), Liverpool School ot 
Tropical Medicine. Memoir XI. 
4. Ibid. 
