TRYPANOSOMIASIS EXPEDITION TO THE CONGO 77 
parasites were seen in their blood for a few days (about twenty-four to a cover). 
Since March 20 these animals have been negative. 
Rabbits. — Incubation period from rive to fifteen days ; average from seven to 
nine days. Duration from fifty to one hundred and twenty -eight days, in some 
cases the organisms have been fairly constant in the peripheral blood, in others they 
appear and disappear at irregular intervals, and the numbers vary from a few to twenty 
to a field. In these animals loss of weight and diminution of red cells and haemo- 
globin are constant features of the disease. In only one rabbit, which lived one 
hundred and twenty-eight days, have we noticed a purulent discharge from the nose, 
eyes, etc., with loss of hair, and then only m the last six weeks of its existence. 
Cats. — Two experiments, both inoculated intravenously. Experiment 422, 
inoculated March 12 from a mouse. Trypanosomes were observed on March 19. 
Parasites were constantly present, the numbers usually being small. The temperature 
rose on March 20 to 104-4° F. an d 011 the 22nd to 104-6°. Some loss of weight 
and anaemia have been observed. Experiment 447, inoculated March 22 from a 
rat ; positive on March 26. Parasites were constantly present and in fair numbers — 
often one to each field. No rise of temperature was observed. 
Puppies {from three tj seven mouths). — Three experiments. Incubation periods, 
five days after intravenous and ten days after intraperitoneal and subcutaneous 
inoculation. Duration from thirty-three to forty-three days. In two cases the 
parasites were to be found almost constantly and in fair numbers which increased in 
the later stages of the disease ; before death the blood became negative again. In 
the remaining experiment trypanosomes were only to be found at intervals and in 
small numbers. Loss of weight occurred in all three, and in two cases there was a 
marked diminution in the number of red corpuscles with a corresponding decrease in 
the percentage of haemoglobin. Subnormal temperature was recorded 111 one case 
before death. 
Goat. — Small female, Experiment 518. Inoculated mtrapentoneally on April 
14 from a rat showing numerous trvpanosomes. Strain ' Lammin.' Parasites 
appeared in the blood on April 21, but have not been found since. No rise of 
temperature nor other symptoms have yet been observed. 
Donkey. — Inoculated subcutaneousiy on February 23 from a rat. Positive on 
March 9. Parasites were found on rare occasions, and then only in very small 
numbers. There was no definite rise of temperature. Some diminution in the 
percentage of haemoglobin and the number of red cells has been observed. 
Monkeys. — Macacus rhesus, Experiment 311. This monkey had been infected 
by Dr. H. E. Annett with blood from the European Case H. K.' Parasites were 
found only at intervals and in small numbers, though the animal appeared to be very 
1. H. E. Annett, First Report of the Trypanosomiasis Expedition to Senegambia, 1902, Liverpool School of Tropical Medicine, 
Memoir XI, p. 4. 
