78 THOMPSON YATES AND JOHNSTON LABORATORIES REPORT 
ill and emaciated ; at the end of nine months it had quite recovered. In January, 
1904, rats inoculated from it during the preceding four months not having become 
infected we decided to attempt to reinfect the animal. On January 29, thirteen 
months after its original infection, it was inoculated with blood from a rabbit 
('Gunjur' strain). On February 6 parasites were found in the blood ; they were 
almost always present, though not in great numbers, up to March 24, from which 
date until death, on April 3, the blood remained negative. Very marked oedema of 
both upper and lower eyelids, intraorbital space, and eyebrows occurred on March 27 ; 
this persisted for three days ; no parasites were to be found in the oedema fluid. No 
other symptoms other than loss of weight and anaemia were observed. 
Macacus rhesus, Experiment 517. Inoculated intraperitoneally on April 14 from 
a rat showing numerous trypanosomes. Strain ' Lammin.' Parasites appeared in 
the blood on April 16. Death occurred on April 18 from purulent and haemorrhagic 
peritonitis caused by a parasite found in the intestinal walls and not yet identified. 
A chimpanzee, Experiment 134, 1 inoculated by Dutton and Todd in July, 1903. 
Parasites were rarely seen in the blood, and only in small numbers. After the third 
week in September trypanosomes were found only once in the blood, on January 27, 
when one was seen. On this date the temperature rose from its usual level of 
between 99 0 and ioo° to I03 , 6°F., falling again next day. Parasites were not seen 
again before death, which occurred from broncho-pneumonia on February 10. 
Sub-inoculations. — Two mice and a rat inoculated on November 20 did not 
become infected. Of the two mice and a rat inoculated on November 27 the mice 
did not become infected, while the rat was positive once, January 7, when one 
trypanosome was found in its blood. Two mice and a rat inoculated with large doses 
of heart blood immedately after death never became infected. 
Symptoms. — There were occasional rises of temperature to 102 0 to I03°F. with 
no apparent cause. One of these occasions (November 27) one trypanosome was 
found in the blood. There were temporary loss of weight and appetite and 
falling off in condition towards the end of December, 1903. The animal subse- 
quently improved and regained appetite. A blood count in July, 1903, showed 
haemoglobin 85 per cent, and red cells 8,360,000 per cubic millimetre. One made 
on February 6, 1904, showed haemoglobin 60 per cent, and red cells 4,540,000 per 
cubic millimetre. 
Horse. — Experiment 87. The small bay stallion 2, inoculated in West Africa by 
Dutton and Todd in February, 1903, and brought to England in July, 1903, is alive 
and in good condition, and seems to have recovered. Trypanosomes have not been 
seen in its blood since it was brought to England. Two rats inoculated from it at 
the end of July became infected. Rats and mice inoculated on October 2 and 
1. Dutton and Todd, loc. cit. 
2. Dutton and To Jd, loc. cit. 
