SUPPLEMENTARY NOTES ON THE TSETSE-FLIES 
Larva of Glossina palpalis 
I have been enabled to examine a series of sixty-two larvae of this species, ranging 
in length from 2 mm. to 7 mm., but only one of these (the largest) can be regarded 
as adult. The colour of these specimens, which have been preserved in 5 per cent, 
formalin, varies from cream to buff yellow. In all the larvae the tumid lips on the 
last segment, as seen in the pupa, are plainly visible, and in a lirva of 2^ mm., which 
may perhaps be considered to be in the first stage (i.e., before the first moult), the 
size of the lips is relatively, if not even actually, considerably greater than in a larva 
measuring mm., which is perhaps in the second stage (i.e., between the first and 
second moults). In the former stage, too, the lips are much wider apart than in the 
second, and the very young larva viewed from above may be said to be conical in shape, 
with a protuberance on each side of the truncate posterior extremity. In the second 
stage (larvae from 3 mm. to 3^ mm. in length) the lips are nearer together, and 
separated by a much narrower interval than in larvae in the third stage (about 3-j mm. 
in length and upwards), in which they have their final position, as seen in the pupa. 
In the first stage the lips, or anal protuberances, appear slightly brownish all over ; in 
the second stage they are blackish at their extremity and at the margin of the inter- 
vening notch ; in the third stage they are uniformly deep black, and the granules with 
which they are covered can now be easily discerned under an ordinary platyscopic lens. 
In the second and third stages the body of the larvae in front of the bifid anal extremity 
(twelfth segment) is seen to be composed of eleven clearly marked segments. In the 
larger larvae the tips of the mouth hooks can be seen slightly protruding from the 
cephalic end. The larva already referred to as being the only one that can be 
regarded as adult, measures 7 mm. in length, by 3 mm. in greatest width, and was 
obtained by Captain Greig, at Entebbe, Uganda, in April of the present year. In 
this larva, in the median ventral line, each of the segments from the fourth to the 
tenth shows on its anterior margin a narrow ridge, measuring about § mm. in trans- 
verse width. The object of these ridges may be to assist the adult larva on extrusion 
to crawl away to some hiding place in which the pupal stage may be assumed. 
All of the larvae here referred to were deposited by the parent flies in boxes, and, 
since all, with a single exception, are immature, it follows either that Glossina palpalis 
differs from the species described by Colonel Bruce, in that the larva feeds and grows 
outside the body of the mother after extrusion, or else that the parents of these larvae, 
probably owing to their being in captivity, gave birth to their offspring prematurely. 
The latter supposition would appear to be the more reasonable. 
Pupa of Glossina palpalis 
The pupae examined were all obtained at Entebbe, Uganda — those forwarded by 
Dr. Nabarro in September, 1903, while others were collected in April, 1904, by 
Captain Greig. Additional specimens were sent home last year by Colonel Bruce, 
