TRYPANOSOMES, TRYPANOSOMIASIS, AND SLEEPING SICKNESS 13 
both obtained by him from Col. Bruce. A reply to the publication by Plimmer 
has already been made. 1 In correspondence with Professor Laveran, who has also 
experimented with both of the strains used by Mr. Plimmer, he writes that his 
results are equally opposed to Plimmer's. In order that Mr. Plimmer's findings 
may be compared with our results, a synopsis of his report is printed. 
Further experiments and comparisons have been made with the various strains 
of trypanosomes. We have been able to successfully inoculate four baboons, one 
Cynocephalus sphinx, three Cynocephalus babuin, with strains of T. gambiense ; these 
animals have all died. The baboon is certainly the most resistant animal with which 
we have experimented. From our baboon experiment, No. 747, a highly virulent 
strain has been recovered by passage through a rabbit. 
Baboons {Cynocephalus babuin) 
Experiment No. 709. Inoculated intraperitonealiy July 24, from Rhesus, 672. 
Mixture contained two to six trypanosomes to a field. The monkey, 672, was a 
direct inoculation. On August 10 a temperature of 104-6° F. was registered ; no 
parasites were seen. The animal lost weight, and the blood counts showed a 
diminished number of red corpuscles and of haemoglobin. No parasites were seen up 
to death, October 7. During the ten days preceding death the animal became very 
emaciated, and for the last thirty-six to forty-eight hours weakness was marked. 
Baboon lying down and almost unconscious. A rabbit inoculated with nearly ten c.c. 
of heart blood after a greatly prolonged incubation became infected. Up to date it 
has never shown parasites in large numbers. 
Experiment No. 747. Inoculated intraperitonealiy August 21, the temperature 
rose on the sixteenth day and after that date continued very irregular — I04 , 2° F. being 
frequently registered. Death occurred on October 10, being hastened by a severe 
dysentery which commenced five days before. Parasites were first seen on October 1 
and again on October 4, each occasion a high temperature was registered. In this 
case, as also in the other two, though the blood was so often or always negative still 
the characteristic clumping or autoagglutinatioir of the corpuscles was pronounced. 
This phenomenon occurred gradually but was easily determinable about the eleventh 
to fourteenth day after inoculation ; it persisted to the end. At the autopsy, which 
was done immediately after death, a rabbit, 823, and two guinea-pigs were inoculated 
with large amounts of blood which was negative even when centrituged. The rabbit 
littered on October 25, at the same time the temperature rose, the autoagglutination 
of the corpuscles was present but no parasites were seen. On November 1, 105*1° F. was 
registered ; the next day, the twenty-third after inoculation, parasites were seen ; these 
increased very rapidly so that thirty to forty to a field were present. From this rabbit 
I. Proc. Roy. Soc, 1905. 
2. Dutton and Todd. 
