16 THOMPSON YATES AND JOHNSTON LABORATORIES REPORT 
Donkeys 
Two were inoculated, one with 'Gunjur' strain (Lancet, May 14, 1904), the other 
with Uganda sleeping sickness strain, between February and March, 1904. These 
animals became infected, and showed the parasites in their blood. The parasites have 
always been extremely scanty in numbers, even after centrifuging the blood. The 
occasions on which the blood examination has been negative have gradually become 
more frequent, but they are still occasionally seen. The autoagglutination of the 
corpuscles is marked. Few symptoms have been observed. For about two months 
after infection, the animals appeared to be losing flesh and were less lively, they have 
now regained their normal weight. Anaemia has been present from the commence- 
ment but it is not marked. The temperature has continued irregular, varying 
between 98-6° and ioo - 4° F., occasionally it rises to ioi'8° to io2 - 4° F. about every 
ten to fourteen days or so. This rise continues for about forty-eight hours, when 
the temperature drops to normal or sub-normal. The urine is negative. Glandular 
enlargement is present in both these animals, it was noticed about a month after 
infection, and still continues, though much less marked. The blood is infective to 
rats in amounts of 0*5 to i-o c.c, formerly a few drops of blood were sufficient. 
Cow 
This animal was inoculated, April 28, 1904, the temperature rose to ioy6° F. 
eight days later, but no parasites were seen until May 17. A rat inoculated on the 
day of the rise, developed the disease. For the first month no symptoms were noted, 
the animal then began to lose flesh, and became quite thin, but in July it commenced 
to put on weight. The yield of milk decreased, the parasites were extremely scanty, 
and have been present in less numbers and at longer intervals than in the case of the 
donkeys and the horse. Anaemia was not marked. The blood count at present is, 
reds, 6,220,000 ; whites, 16,200; haemoglobin, eighty-five per cent. Autoagglutination 
of the corpuscles has been present from May 20 — it still persists, but is not so marked. 
The temperature has shown the same irregularity, rising gradually but irregularly for 
ten days to remain high for about one-and-a-half to three days, and then commencing 
to fall. Glandular enlargement was present up to August, 1904. The coat which 
had become rough and dull looking is now smooth and glossy. The blood is still 
infective though larger amounts are now necessary. 
A peculiar condition has been noted in the milk. About two-and-a-quarter 
months after infection, it was noticed that the milk was very poor in fat, and watery. 
Dogs ted with it developed in about twenty-four to thirty-six hours a very profuse 
diarrhoea, which continued so long as the milk was given. Pasteurising or autoclaving 
at 120 0 C. appeared to make no difference, as pups and kittens developed diarrhoea 
in two to four days. Bacteriologically, the milk has been proved to be free from 
pyogenic organisms. Guinea-pigs inoculated with it have not developed any lesions. 
