TRYPANOSOMES, TRYPANOSOMIASIS, AND SLEEPING SICKNESS 17 
Chemical analyses have failed to detect any changes other than a low percentage of 
fat. Tried on human beings, nothing is noted other than a tendency to cause 
biliousness and diarrhoea. The possibility of antibodies being formed in the milk 
has been recognised, but no proof has been established. 
Sheep 
A wether, Experiment No. 560, became infected ten days after inoculation. On 
the twenty-seventh day one parasite to a field was noted. The numbers gradually 
decreased, and five months later, the animal appeared to have recovered. Blood in 
amounts of ten to forty c.c. was non-infective. Slight anaemia was present for a time. 
Autoagglutination was fairly well marked until the end of the third month. 
Goats 
The animals inoculated with the various strains have all died. One, No. 480, 
inoculated with Uganda sleeping sickness died on the fifty-eighth day. The anaemia 
was somewhat marked. The parasites were present fairly constantly, usually four to 
twenty-four to a cover preparation being seen. Autoagglutination of the corpuscles 
was not very marked. The temperature remained irregular. Loss of weight was 
the only symptom noted. The post-mortem, twelve hours after death, showed a 
slightly enlarged spleen and glands, none of them haemorrhagic. Decomposition 
was advanced. 
Dogs 
These animals, though easily susceptible to inoculation, survive for many months. 
Laveran records one dog still living, six months after infection. A bitch, No. 738, 
inoculated with ' Gunjur ' strain lived for over nine months. The parasites were at 
first hard to find, but for the last three months became more and more numerous. 
For the first two months emaciation was not marked ; a progressive decline then 
became evident, and this continued up to death. Towards the end a profuse dis- 
charge from the eyes occurred ; the conjunctivae were inflamed and infiltrated. 
The blood count of this animal is interesting (p. 1 8). 
Some dogs have died in three weeks, others have lived for varying periods up 
to nine months. A bitch, Exp. 45, brought back by Dutton and Todd, recovered 
from the disease. This animal had been inoculated when a puppy ; it developed a 
chronic form of the disease and at the end of four months no parasites could be 
found in its blood. Autoagglutination of the corpuscles gradually became less 
marked, and ten months after inoculation the blood appeared perfectly normal. In 
amounts of 2-0 to io*o c.c. it was non-infective. Reinoculation of small quantities 
of blood failed to produce infection, although a control dog developed the disease, 
c 
