28 THOMPSON YATES AND JOHNSTON LABORATORIES REPORT 
a loss of four hundred to six hundred grammes is not uncommon in these cases. 
The coat becomes rough. Oedema of the posterior limbs and base of ears may 
occur to a certain extent. Discharges from the eye, nose, and genital orifices are 
rarely seen, and the animal never presents the wretched appearance so often seen in 
rabbits infected with Nagana, Caderas, etc., diseases. Coincident with the appearance 
of the parasites the temperature rises, and after continuing so for twenty -four hours 
falls, after that it may become irregular, being often very elevated, and towards the 
end becoming subnormal. The spleen is enlarged, but not more so than in rabbits 
dying from Caderas, etc. The glands may be somewhat enlarged, a few are often 
haemorrhagic, congestion of the organs is often seen. 
Monkeys 
A Cercopithecus callitrichus, a Macacus rhesus, a Jew monkey, and a baboon, 
Cynocephalus sphinx, have all been successfully inoculated with the disease. The incuba- 
tion in the Calothrix was four days, the duration lasting over one hundred and sixty 
days. The Jew monkey developed the disease on the sixth day, and died seventy- 
five days after inoculation. In both these animals the parasites were never present in 
very large numbers until a few days before death. The Calothrix showed more 
parasites and exhibited more symptoms than did the Jew monkey. Anaemia, loss of 
weight, and irregular temperature were noted in both these cases. A baboon, 
Cynocephalus sphinx, was inoculated intraperitoneally with a large quantity of virulent 
blood from a dog which had died from the disease on February 2 2, 1904. On April 
18 a rise of temperature was noted, but no parasites could be found. On May 12 
eight parasites were found. Unfortunately a daily examination of the blood could 
not be made, therefore no definite incubation period can be given. From that time 
the animal rarely showed parasites in the blood until July 4, when they were nearly 
always present, though in small numbers, up to its death on September 13. The 
temperature was irregular. Loss of weight was very marked, especially when the 
parasites were first seen. Anaemia was not a prominent feature of the disease. A 
Rhesus inoculated with some of the baboon's blood developed the disease on the 
seventh day. Parasites were hardlv ever seen, and it finally died from dysentery. A 
rabbit inoculated intravenously developed the disease after an incubation of eleven 
days, it is still living, and appears to have recovered from the disease. Two guinea- 
pigs inoculated at the same time became infected, but never severely, and died from 
an epidemic of broncho-pneumonia. The serum of this baboon was slightly more 
agglutinative to the parasite than ordinary baboon serum. Its serum was not pro- 
tective. 
Post-mortem. — The autopsy of the Calothrix showed an enlarged firm spleen, 
glandular enlargement was general, but not very marked. The baboon showed a 
slightly enlarged spleen and a little enlargement of the glands. The microscopical 
examination of the brains and organs is referred to by Dr. Breinl. 
