3 o THOMPSON YATES AND JOHNSTON LABORATORIES REPORT 
seen, its blood in amounts of 2 - o to 3-0 c.c. is non-infective. While in England it 
has been bred and its pups used to see if they were less susceptible than other pups 
to trypanosome infection. The results show that they acquire the disease quite as 
easily as other ones, and succumb in the usual time. The serum from the bitch has 
no protective properties. 
Post-mortem. — The spleen is often two to three times larger than normal, the 
substance is dark and friable, sometimes it may be almost deliquescent. The glands 
are enlarged, some may be haemorrhagic. Peritoneal exudate is scanty, the pericardium 
may contain a great deal of fluid. The exudates usually contain a fair number of 
trypanosomes. 
Sheep 
A ram was inoculated intraperitoneally. On the sixteenth day the temperature 
rose slightly and at the same time parasites were seen in the blood. Ten days later 
ten to forty trypanosomes to a field were noted. These continued in large numbers 
for the next seven days. The numbers then fell and remained scanty, though con- 
tinuously present, to death, which took place eighty-four days after inoculation. The 
anaemia was constant and the animal rapidly became thin. Appetite continued good. 
No other symptoms were noted. The animal suddenly died during the night. As 
it was in the height of summer decomposition commenced quickly. The spleen 
was small, the glands were not markedly enlarged. No oedema or petechial 
haemorrhages could be found. 
Goats 
Goat, Experiment 35, inoculated by Dutton and Todd from Horse I, 
November 28, 1902, remained infected up to March 20, 1903, it was then sent to 
England. In August, 1903, its blood was negative to microscopical examination, and 
non-infective even when injected in amounts of 2 - o to 3-0 c.c. It was reinoculated 
in October and became infected. The temperature rose and remained irregular. 
Death occurred after inoculation. The parasites were always very scanty until just 
before death. The autopsy disclosed enlarged spleen and glands, some few of the 
latter being haemorrhagic. Anaemia was noted a few days before death. 
Goat 10, inoculated by Dutton and Todd, October 1 5, 1902. Appeared to have 
apparently recovered in October, 1903 ; in March, 1904, it was killed by accident 
while being bled. A. guinea-pig, inoculated with 100 c.c. of pure heart blood, became 
infected after a prolonged incubation. The serum from these goats did not cause any 
permanent agglutination. 
Horse 
The stallion, 1 naturally infected in the Gambia and sent back by Dutton and 
Todd, has been under almost continuous observation from October 30, 1902. Since 
August, 1903, it has been bled at regular intervals, and its blood inoculated into 
I. First Report of the Expedition lo Senegambia, p. 31. 
