TRYPANOSOMES, TRYPANOSOMIASIS, AND SLEEPING SICKNESS 33 
Rabbits 
Rabbits inoculated subcutaneously develop the disease in six to eleven days and 
die in twenty-four to one hundred and eight days, some have lived over six months. 
The parasites are very scanty during the whole course of the disease. Oedema ot 
the ears and genital organs, very often persisting and increasing, is marked. Loss ot 
hair has been noted on one or two occasions, usually at the base of the ears and 
around the eyes and nose. The genital organs are swollen, the urethra or vaginal 
mucous membrane is pinkish and covered with catarrhal exudate. The discharge is 
often pronounced. The discharges from the eyes and nose are not so marked as in 
rabbits infected with the parasites of Nagana or Caderas. Examination of these 
discharges always proved negative. 
Rats 
Twenty-three rats have been inoculated with large numbers of the parasite ; in 
only two have parasites been found after the fourth day. One died on the eleventh 
day, the other on the eighteenth. In neither case could the autopsy be done early, 
and therefore the negative finding is not to be considered. The spleen and glands 
were small. A rabbit inoculated with the blood and extract of mashed organs ot one 
of these rats never developed the disease. 
Cats 
One adult female cat was inoculated at the same time as a pup ; on the ninth 
day the temperature rose but no parasites could be found. The experiment proved 
negative. 
Guinea-Pigs 
Four have been inoculated with negative results. 
Goat 
A goat inoculated subcutaneously with ten c.c. of heart blood and twenty c.c. of 
peritoneal exudate never developed the disease. Its temperature remained normal, 
and its blood negative ; a pup inoculated from it never became infected. 
Post-Mortem Appearances 
The autopsies on the pups showed a moderately enlarged spleen and enlarged 
glands, some few of which were haemorrhagic, these being found usually in the 
retro-peritoneal, inguinal, and axillary groups. The blood was very serous and 
pale, and usually contained numerous parasites. The bone marrow was pale and often 
very soft. The pericardial sac contained some slightly clouded fluid. The peritoneal 
cavity usually contained a large quantity of clear watery or straw-coloured fluid. 
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