36 THOMPSON YATES AND JOHNSTON LABORATORIES REPORT 
The testicles are generally swollen, there usually is a discharge from the penis or 
vulva. The discharge from the eyes and nose is very severe, causing the animal to 
present a very miserable state. It has been noted that in treated animals the discharge 
and oedema quickly disappear, and the hair begins to grow over the bald patches. 
Guinea-Pigs 
Subcutaneous inoculation causes the parasites to appear in eight to ten days. 
Intraperitoneal injection takes six to eight-and-a-half days. The duration of the 
disease may be from twenty-two to one hundred and fifty-three days, the average 
period being seventy-five to ninety days for adults, and thirty to forty-six days for young 
guinea-pigs (three hundred grammes). The majority of the animals have shown 
parasites in their blood almost constantly ; occasionally the organisms nearly com- 
pletely disappear. This is especially so after a marked increase in numbers. Small 
guinea-pigs usually harbour large numbers ot trypanosomes in their blood. The 
appearance of the parasites in the peripheral blood is coincident with a slight rise of 
temperature. The fever then becomes irregular, but not more so than is met with 
in guinea-pigs infected with Surra, Nagana, etc. Loss of flesh is noted, but is more 
gradual than in rabbits. Paralysis is rarely observed. Three guinea-pigs showed 
partial paralysis of the hind extremities, six, three, and one day before death. No 
guinea-pigs out of forty-three infected and not treated have survived the disease. 
Cats 
Kittens are more susceptible than adults. The incubation is six-and-a-half to 
eight days, and death occurs in about thirty to fifty-six days. Adults develop the 
disease in seven to nine to eleven days, and may die in two-and-a-half to six months. 
The appearance of the parasites in the general circulation is associated with a rise of 
temperature. The chart of an infected animal showed an irregular rise and fall with 
sharp rises to 104 0 to 105 0 F. With kittens the temperature usually remains high. 
The adults rarely show the parasites in their blood, but the agglutination of the 
corpuscles is often very pronounced. Kittens usually have the parasites almost con- 
tinuously in the peripheral circulation. The parasites usually are found in the blood 
at the time of the sharp rises of temperature. Oedema is rarely observed. Dis- 
charges from the eyes, nose, and genitals are rare. The autopsy shows a certain 
enlargement of the spleen. The glands are somewhat swollen. Haemorrhagic 
glands are rarely seen. In the kitten the gland juice frequently contains many 
parasites. 
