4 o THOMPSON YATES AND JOHNSTON LABORATORIES REPORT 
V. BACTERIOLOGICAL EXAMINATIONS, MORPHOLOGY, GLAND 
PUNCTURES, AND BLISTERS 
Bacteriological Examinations 
Cultures of the blood and cerebro-spinal fluid were made from two of the fatal 
cases of sleeping sickness and several times from ' Native Fever ' cases. 
The blood was obtained from one of the arm veins by means of a Roux Syringe 
— the cerebro-spinal fluid through lumbar puncture. The media used consisted of 
broth, agar and gelatine of various alkaline or acid reactions. Sugars or peptone, salt, etc., 
were contained in some media, in greateror less proportions, and were absent from others. 
Large Ehrlenmeyer flasks containing fifty to two hundred c.c. of nutrient media were 
inoculated with some of the blood or cerebro-spinal fluid. Anaerobic and aerobic 
methods of cultivation were employed. The inoculated media were incubated at 
1 8°, 23 0 , and 35 0 C. 
From Kitambo. Cerebro-spinal fluid, one flask and two tubes showed small 
growths of Staphylococcus epidermidis albus. These were the first cultures inoculated. 
From Tomi. All cultures remained sterile. All cultures were kept under 
observation for two months. 
From some of the animals infected with the cerebro-spinal fluid of the two 
sleeping sickness cases cultures were made at death, the results were negative. 
At the post-mortem on the two sleeping sickness cases, cultures were made. The 
blood and cerebro-spinal fluid cultures remained negative. The spleen and liver 
showed in Kitambo B. colt communis and putrefactive bacteria. 
From Tomi. No growth. 
Variations in the Morphology of the Parasites 
From a comparison of stained preparations of T. gambiense made during the 
course of the disease no differences can be made out. The parasites in the sub- 
inoculated animals seemed somewhat larger. This seemed to be the case with many 
trypanosomes. 
The parasites in the three cases of Trypanosome Fever compared with those 
found in the blood of the two sleeping sickness cases show no determinable 
differences. The organisms found in the cerebro-spinal fluid of one case showed a more 
vacuolic condition, the parasites appeared slightly more rounded at the posterior end. 
None of the amoeboid forms described by Castellan 1 were seen. The trypanosomes 
in the blood of the same case did not show the vacuoles so markedly. The 
observance of many slides of blood, which is often very serous, and of exudates, 
