56 THOMPSON YATES AND JOHNSTON LABORATORIES REPORT 
trypanosomes were seen at the end of a day. The parasites were absent from the 
blood ; 2 - o grammes of atoxyl was given twice a week. The animal was very anaemic, 
emaciated, and weak. It improved for a time. Unfortunately, our early departure 
prevented us making a study of this case, and, moreover, as there was no data as to 
the dose of the drug the administration had to be cautiously proceeded with. 
T. evansi. — The results in the small laboratory animals are about the same as 
with Nagana. The animals have lived as long. 
A horse was injected subcutaneously with blood from an infected rat ; many 
divisional forms seen. Incubation four days ; temperature rose on fifth day and 
parasites rapidly increased in numbers ; the animal eat little and stood with head 
hanging down. The numbers increased to forty thousand to the c.mm.; the 
haemoglobin fell from ninety-five to sixty-three per cent. ; no great change was noted 
in the red and white blood cell count. When definite loss of weight and anaemia had 
occurred and the parasites were about five hundred to the cover, i*o gramme of atoxyl 
in the form of a ten per cent, solution was given subcutaneously ; in thirty hours 
the parasites had disappeared. A slight leucocytosis was observed ; 2 - o grammes 
were then given and this was continued twice a week. The animal grew fatter and 
the blood count improved. 
T. equinum. — Guinea-pigs react to treatment quickly and for a considerable time, 
but there is a tendency for some of them to show parasites in their blood one and two 
months after treatment has stopped. On the other hand, one treated for two-and-a- 
quarter months survived eight-and-a-half months after inoculation, treatment being 
stopped for two-and-three-quarter months. This animal was killed, and rats and guinea- 
pigs inoculated with its blood have never become infected. A rabbit which had been 
infected for twenty-eight days showed the loss of hair around the eyes and nose, and 
the purulent discharge from the eyes, nose, and urethra. The oedematous condition of 
the ears and genitals has by treatment with i - o c.c. of ten per cent, solution once 
every five days disappeared so far that all the external signs are wanting and no 
parasites are found ; it has, up to date, lived seventy-six days. 
T. equip erdum. — The work has been entirely on pups ; these parasites have 
reacted in the ordinary manner, but the tendency of young puppies to manifest toxic 
symptoms has resulted in the experiments only prolonging the disease for one to two 
weeks longer than their controls. A bitch, inoculated January 14 ; positive 
January 20 ; large number first appeared on the 26th, temperature markedly 
irregular. Periodicity noticed ; usually parasites absent for one to three days, then 
a rapid increase. Animal became very anaemic and thin. The blood count before 
inoculation gave 5,860,000 reds; 11,000 whites; and ninety-seven per cent, 
haemoglobin. On February 25 the reds were 3,780,000 ; whites, 14,000 \ 
haemoglobin, sixty-five per cent. ; parasites were six to eight to a field. Trypanroth 
sixteen c.c. one per cent, solution administered. In thirty-six hours no parasites 
