TRYPANOSOMES, TRYPANOSOMIASIS, AND SLEEPING SICKNESS 71 
seen not far from the capsule, rilled with lymphocytes, and surrounded by red blood 
corpuscles, these latter being easily distinguishable from the blood vessels by the 
absence ot endothelial cells. 
The cellular elements included divisional forms of lymphocytes ; giant cells, 
resembling those of bone marrow ; large hyaline cells with included red corpuscles 
in all stages of degeneration, sometimes only appearing as vacuoles ; mono- and 
polymorphonuclear leucocytes with eosinophile granulation ; phagocytes ; and 
plasma cells. 
In some of the glands were seen all stages between phagocytes containing red 
cells and eosinophile cells. In the first stage were phagocytes with their nucleus placed 
peripherally, and the protoplasm of the cell filled with red corpuscles, stained a 
peculiar colour with eosine. The eosine-stained red corpuscles became smaller and 
smaller in their including cells, whose protoplasm in a later stage seemed to be 
unevenly stained a brilliant red tinge. Later, the nucleus changes its form and 
becomes polymorphonuclear, and in other cells one can almost see the eosinophile 
granules crystalizing out from this red-stained protoplasm. Some of the eosinophiles 
are destroyed in the lymph glands by phagocytosis, whilst others appear to enter the 
circulation. New formation of lymphatic tissue was noted ; for example, in the 
adipose tissue around the axillary glands there was an accumulation of lymph cells 
between the fat cells, and in some places there was distinct follicle-like formation 
around the vessels. 
The Bone Marrow of the femur in some places had normal appearance ot tat 
marrow, but in others however the marrow was very cellular, the fat tissue being 
reduced to a few islets, and replaced by cellular tissue composed of normal marrow 
cells with very few nucleated red corpuscles, and a large number of eosinophile cells. 
The vessels were greatly congested and there were frequently haemorrhages around 
the vessel walls ; the blood corpuscles showing degeneration. In different parts there 
were varying numbers of phagocytes containing red blood cells. Blood pigment 
was only infrequently met with. Here and there the whole of the tissue was replaced 
by a homogenous substance containing very few cells, staining yellowish with van Gieson 
and red with eosine, and presenting the appearances of gelatinous degeneration. 
Brain and spinal cord were fixed in tour per cent, formalin and hardened in alcohol 
and sections were cut from the different regions. The sections were stained by 
haematoxylin, eosin and van Gieson, W eigert's method, Weigert-Marchi, Nissl and 
Unna, and for bacteria with Loffler's methylene blue, carbolfuchsin, Pfeiffer, carbol 
thionin and Gram. 
The pia and arachnoid shewed a fair amount ot pigmented cells, similar to those 
observable in skin, the pigment being dark-brown and granular. The large and small 
vessels were surrounded in a varying degree by small-celled infiltration ; sometimes 
the larger vessels had a quite normal wall. The small-celled infiltration consisted of 
