ioo THOMPSON YATES AND JOHNSTON LABORATORIES REPORT 
were present in the few drops of fluid drawn by a hypodermic syringe from the enlarged 
glands of infected persons ; that parasites could be frequently demonstrated by this 
method where careful centrifugalizing of peripheral blood had failed ; and that the 
parasites were often seen in as great numbers in the preparations of gland fluid as in 
the preparations of the sediment obtained by centrifugalizing many (twenty) cubic 
centimetres of cerebro-spinal fluid. 
These results were most encouraging, and it was resolved to test the method 
further by using it in the examination of apparently healthy persons. Twenty-two 
natives, coming from districts where the disease occurred, but all absolutely unsuspected 
of being cases of ' sleeping sickness,' were chosen because of their more or less 
enlarged glands. These were punctured and trypanosomes were found in the fluid 
aspirated in eight instances. A simultaneous examination of fresh coverslip preparations 
only detected two of these cases, and by centrifugalizing the blood only one additional 
case was revealed. Lumbar puncture was done on two of the cases found to be infected 
by gland puncture and the spinal fluid shown to be in every way normal. Although 
the largest cervical glands have usually, for convenience sake, been those punctured, 
parasites have been found, with equal facility, in glands no larger than peas and in 
glands from other groups. 
Only twice during the examination of a series of thirty cases of trypano- 
somiasis did a single examination of gland fluid fail to demonstrate the parasite which 
other methods of examination showed to be present. 
On seven occasions (in twenty-six cases) it was successful when centrifugalizing the 
blood had failed, and it was thrice positive (sixteen cases) where examinations of the 
cerbro-spinal fluid had given negative results. 
Although a general glandular enlargement is very common among African 
negroes, it seems possible that, at all events in infective areas, persons with much 
enlarged lymphatic glands must, other causes being absent, be regarded as possible 
cases of trypanosomiasis. For example, fresh coverslip preparations of blood from a 
squad of twenty-six healthy soldiers were examined. Two men were infected. Both 
had been previously chosen, with three others, for gland puncture because of their 
enlarged cervical glands. Unfortunately, further examination of these cases was not 
permitted. 
A small herd of cattle was established near Coquilhatville in 1902 by the Congo 
Government. The cattle suffered a good deal from an unrecognized chronic 
wasting disease, ending in death. The veterinary surgeon (M. Bertolotti) in 
charge had made an excellent report to the Governor upon the clinical aspects of the 
disease, and there seemed to be some analogy between the symptoms which he 
described and those observed in Gambian horses and cattle infected naturally, or by 
inoculation, with T. dimorphon. x 
When we arrived in Coquilhatville only one cow out of a herd of forty odd 
