ELEMENTARY COMPOSITION OF OXYHEMOGLOBIN 199 
The subsequent researches of Hoppe-Seyler soon demonstrated, 
however, that the blood crystals obtained from the blood of different 
animals did nol possess an identical composition, though the differences 
brought out by Eoppe-Seyler's analyses were nol very great. His 
results are shown in the table on p. 198. 1 
The very numerous analyses of oxyhemoglobin of different animals, 
made in recent years by Kossel, 2 Otto, 3 Zinoftsky, 4 Hiifner, 5 Jaquet, 6 
and others exhibit, however, such extraordinary discrepancies in the 
results of ultimate organic analysis as to preclude a precise answer 
being given to such simple questions as the following: — 
Is haemoglobin a body, having a constant composition in animals 
of the same species : 
Does the haemoglobin of different animals vary in chemical com- 
position, and if so, within what limit - ' 
Results of the more recent Analyses of Oxyhoemoglobin (1878-1890). 
Oxyhemoglobin of 
c. 
H. 
X. 
S. 
Fe. 
O. 
P 2 5 . 
Dog . 
53-85 
7-32 
16-17 
0-39 
0-43 
21-84 
Il"ppe-Seyler. 7 
j, 
53-91 
6-62 
15-98 
0-540 
0-333 
22-62 
Jaquet. 8 
Jl 
54-57 
7-22 
16-38 
0-568 
0-336 
20-93 
Jaquet. 9 
Horse 
54-87 
6-97 
17-31 
0-650 
0-47 
19-73 
Kossel. 10 
j, 
54-76 
7-03 
17-28 
0-67 
0-45 
19-81 
Otto. 11 
j> 
54-40 
7-07 
17-40 
0-66 
0-45 
19-74 
Biieheler. 12 
51-15 
6-76 
17-94 
0-39 
0-335 
23-43 
Ziuoffskv. 13 
Ox '. '. '. 
J , * 
54-66 
7 -25 
17-70 
0-447 
0-400 
0-336 
19-54 
Hiifner. 14 
Hiifner. 13 
Pig . 
54-17 
7-38 
16-23 
0-660 
0-430 
21-360 
Otto. 16 
54-71 
7-38 
17-43 
0-479 
0-399 
19-602 
Hufner. 17 
Hen! '. '. 
52-47 
7-19 
16-45 
0-857 
0-335 
22-500 
0-197 
Jaquet. 18 
1 Med. Chem. Untersueh., Berlin, 1868. Heft 3, S. 370. 
2 The results of the analyses made by Dr. Kossel were published in a paper by Hoppe- 
Seyler, entitled "Weitere Mittheihmgen ueber die Eigenschaften des Blutfarbstotfs — Das 
Oxyhemoglobin des Pferdeblutes, " Ztschr. f. physiol. Chem., Strassburg, 1878-79, Bd. ii. 
S. 149-155. 
3 "Ueber das Oxyhemoglobin des Schweines," ibid., 1882-83, Bd. vii. S. 57-68. 
4 " Ueber die Grosse des Hamoglobinmoleciils," ibid., 1SS6, Bd. x. S. 16-34. 
5 "Ueber das Oxyhemoglobin des Pferdes," Ztschr. f. physiol. Chem., Strassburg. 
1883-84, Bd. viii. S. 358-365; " Beitriige zur Lehre voni Blntfarbstoffe," in Beitr. 
Physiol. C. Ludwig z. s. Geburtst. etc., Leipzig, 18S7, S. 74-81; "Neue Versuche zur 
Bestimmung des Sauerstoffscapacitat des Blutfarbstotfs, " Arch. f. Physiol., Leipzig, 1894 
S. 130-176. See especially S. 174-176. 
u " Elementaranalyse des Hundeblut-Hamoglobins," Ztschr. f. physiol. Chem., Strass 
burg, 1888, Bd. xii. S. 285-288 ; Beitriige zur Kenntniss des Blutfarbstoffes," ibid. 
1890, Bd. xiv. S. 289-296. 
7 This analysis, which is adduced for purposes of comparison, does not fall within the 
dates given above, having been published in 1868. 
8 Ztschr. f. physiol. Chem., Strassburg, 1888, Bd. xii. S. 285. 
9 Ibid., 1890, Bd. xiv. S. 2S9. 
10 Ibid., 1878-79, Bd. ii. S. 149. Refer to Note 2. 
11 Arch. f. d. ges. Physiol, Bonn, 1SS4, Bd. xxxi. S. 240. 
12 Hufner, Ztschr. f. physiol. Chem., Strassburg, 1883-84, Bd. viii. S. 358. This paper 
contains the results of Biicheler's researches, which have been carried out under Hiifner's 
direction, and had appeared as a Tiibingeu Dissertation in 1883. 
13 Ibid., Strassburg, 1886, Bd. x. S. 16. 
14 Beitr. z. Physiol. C. Ludvngz. s. Geburtst. etc., Leipzig, 1887, S. 74. 
15 Arch. f. Physiol, Leipzig, 1894, S. 174. 
16 Ztschr. f. physiol Chem., Strassburg, 1882-83, Bd. vii. S. 57. 
11 Beitr. z. Physiol. C. Ludwig z. s. Geburtst. etc., Leipzig, 18S7, S. 74. 
18 Ztschr. f. physiol. Chem., Strassburg, Bd. xiv. S. 289. 
