CONNECTION BETWEEN BL OD AND ITS GASES. 7 6 7 
oxygen, measured al and 760 mm., which can be absorbed by one 
volume of water. 
Bohr 1 found thai the absorption coefficient of oxygen in a 2 per 
cent, aqueous solution of haemoglobin at 15° was 002249, whereas that 
for pure water is, according to Winkler, 003415, or 50 per cent, greater 
at the same temperature. 
It has been shown that arterial blood with a temperature of 37 c 
contains a large quantity of oxygen, about 22 volumes per cent., an 
amount which could not be present in simple solution. Further, when 
the red corpuscles are absent, as in plasma and serum, the amount of 
oxygen in the fluid is. according to Pfluger, 2 only 0"2<j vols, per cent. 
In the next place, different observers have shown that crystals of 
haemoglobin can absorb large quantities of oxygen. 
Condition of Haemoglobin. 
Amount of Oxvgen 
(0° and 760 Mm.). 
Observer. 
100 grms. 
moist crystals ..... 
108-4 c.c. 
Hoppe-Seyler. 3 
,, 
crystals dried with filter-paper . 
76-9 ,. 
;> 
: 5 
crystals dried at 0' and powdered 
54-2 ,, 
)> 
,. 

156-6 .. 
Dybkowsky. 4 
" 
,, from horse .... 
44-83- 
88-52 ,, 
180-3 ,, 
172-4 ,, 
Strassburg. 5 
Preyer. 6 
n 
,, 
128 
Worm Midler. 7 
j j 
159 
Hiifner. 8 
" 
Mean of 10 determinations. 
crystals dried at and powdered, 
from ox-blood .... 
134 
Hiifner. H 
The causes of these differences are various ; the crystals of 
haemoglobin were prepared in different ways, and it is prohable that 
in some cases methaemoglobin or other products were formed ; the 
amount of moisture varied, and the methods employed for the extraction 
of the oxygen were different. 
Bohr, 10 however, would explain these differences in another manner, 
for he maintains that there are at least four different kinds of haemo- 
globin, a-, &-, -/-, and ^-haemoglobin, which have the same spectrum, but 
combine with different amounts of oxygen, 0*4, OS, 1'7, and 27 c.c. 
Exp. Untersuch. u. d. Sauerstoffaiifnahme des Blutfarbstoffes," Copenhagen, 18S5, 
S. 37. 
- Arch./. d. ges. Physiol., Bonn, 1868, Bd. i. S. 73. 
:; Virchow's Archiv, Bd. xxix. S. 598 ; Med.-chem. Untersuch., 1867, Bd. ii. 8. 
4 Hoppe-Seyler, Med.-chem. Untersuch., 1S66, Bd. i. S. 117. 
5 Arch./, d. ges. Physiol., Bonn, 1871, Bd. iv. S. 454. 
6 Centralbl. f. d. med. Wissensch., Berlin, 1866, Ko. 21. 
7 Ber. d. k. sdchs. Gesellsch. d. Wissensch., Leipzig, 1S70, Bd. xxii. S. 351. 
8 Ztschr. f. physiol. Ch, .. Strassburg, 1878, Bd. i. S. 317, 386. 
9 Arch. f. Physiol., Leipzig, 1894, S. 130. 
10 Skandin. Arch./. Physiol., Leipzig, 1892, Bd. iii. S. 47, 69, 76, 101. 
191. 
