ENDOTHIA PIGMENTS 
Experimental 
For the purpose of obtaining a sufficient quantity^ of pigment for in- 
vestigation, Endothia fluens was grown in flasks on sterilized rice. The 
culture medium with the mycelium was dried and ground, and the coloring 
matter obtained in the manner described by Hawkins and Stevens. The 
method is briefly as follows : 
A cold alcoholic extract of the powder was evaporated under vacuum 
Fig. I. Photomicrograph of crystal aggregates of endothine red deposited from water. 
until most of the alcohol had been removed, and the residue, which con- 
sisted of a mixture of the impure yellow and red substances, was treated 
with a large volume of water. This treatment caused a heavy reddish- 
yellow precipitate to separate, leaving the supernatant liquid dark red. 
A smaller amount of the red compound was recovered from the mother 
liquor. The reddish-yellow precipitate after thorough extraction with 
ether left a residue which consisted mostly of endothine red in a semi- 
crystalline state. By repeated recrystallization from alcohol it was finally 
obtained comparatively pure. It was possible, also, to purify the pigment 
by dissolving it in boiling water and allowing it to stand for several days. 
« The author is indebted to Dr. Hawkins for most of the crude material used in this 
investigation. 
