4o8 
J. L. WEIMER 
It will be seen from the above table that the spore size is rather con- 
stant regardless of the medium upon which the fungus grows. The maxi- 
mum and minimum limits for all the measurements are 9-13 x 13-19 
compared with lo-ii x 13. 5-16 11 as given for P. curvicolla by Grifhths, and 
with 14 as given by Winter. 
Secondary Appendages 
The presence of secondary spore appendages has not been satisfactorily 
demonstrated in this strain. The primary appendage is usually clearly 
evident on young and recently matured spores, but the examination of 
thousands of spores in all stages of development has failed to convince the 
writer of the presence of secondary appendages. However, in the cases of 
a half dozen spores a small fragment of something which resembled a second- 
ary appendage was noticed at the end of the primary appendage, although 
none has ever been seen at the opposite end. This may have been the 
remnant of the secondary appendage, or it may have been some foreign 
matter or protoplasm from the ascus which had assumed a shape resembling 
a short appendage. In the instances noted the projections observed were 
not long and whip-like as described for the appendages of P. curvicolla, 
but it is entirely possible that they had been broken or had deliquesced, 
since they are said to be "very fugacious." 
It seems possible that the presence or absence of these secondary ap- 
pendages may also depend upon the environmental conditions under which 
the fungus developed. However, this could be determined with certainty 
only by long and careful study of many strains of the organism grown under 
various conditions and subject to different stimuh. 
Size of Perithecia and Asci 
It has been seen that the spore size compared very closely with the 
measurements given for P. curvicolla, the maximum and minimum limits 
including those of the latter. A somewhat greater variation in the size of 
Table 2 
Medium 
Age of Cul- 
ture 
Receptacle in which 
Grown 
Perithecial Size Max. and Min. Limits. 
15 days 
Test tube 
400-572 X 614-786 IX 
15 days 
Test tube 
529-572 X 815-929 /i 
String bean agar 
14 days 
Petri dish 
343.2-513 X 472-629 M 
Irish potato agar 
56 days 
Petri dish 
472-686 X 858-1001 IX 
Irish potato agar 
II days 
Petri dish 
430-458 X 595-644 M 
Irish potato agar 
30 days 
Petri dish 
429-543 X 544-829 M 
41 days 
Erlenmeyer flask 
200-429 X 743-1 lOI 11 
33 days 
Test tube 
400-600 X 715-1004 IX 
200-686x472-1101^1, max. 
and min, limits 
400-547 x 669-868 IX averages 
