GERMINATION AND DEVELOPMENT OF EMBRYO OF ZEA MAYS 449 
Plants Developed from 
Yellow Dent 
White Dent 
White Flint 
Kernel 
Embryo 
Kernel 
Embryo 
Kernel 
Embryo 
Leaves (number) 
5 
4 
5 
4 
5 
4 
Length of mesocotyl and leaves 
25 cm. 
13 cm. 
25 cm. 
13 cm. 
30 cm. 
17 cm. 
Length of mesocotyl 
4 cm. 
0.5 cm. 
3 cm. 
I cm. 
3 cm. 
I cm. 
Length of principal root 
25 cm. 
8 cm. 
20 cm. 
12 cm. 
30 cm. 
15 cm. 
Secondary roots (number) 
4 
3 
4 
4 
4 
3 
July a few of the plants had attained a height as great as that of the plants 
developed from kernels. 
The first tassels appeared in July 23 on one stalk in each row of both 
embryos and kernels in the flint strain. Generally the appearance of tassels 
and silk followed the individual vigor and development of stalks rather 
than the ordinary behavior of each of the strains. The tasseling period was 
extended from July 23 to August 10. The appearance of the silk and also 
the pollination, as well as the whole vegetative development, were hindered 
by the storm of August 8 and by the cold weather which followed for a few 
days afterward. On account of adverse weather conditions, the silk, like 
the tassels, appeared irregularly, and especially it was noticed that on a 
few stalks which were beaten down by the storm the silk appeared late, 
after the pollen had been entirely shed. All the plants were in the pro- 
terandrous condition. 
During the time of pollination careful observations were made in re- 
gard to the vitality of the pollen. The writer was not able to detect any 
difference in size, shape, and especially in the vitality of the pollen produced 
by the plants developed from the embryos and by those produced from 
kernels. The vitality of the pollen was tested by artificial pollination and 
by germination of the pollen in a solution of 10 per cent sucrose and 0.75 
percent agar (i). 
Each stalk produced at least one ear, and there was only one barren 
stalk which appeared in the row of the Yellow Dent variety developed from 
the embryo. 
On account of the cold weather the maize was harvested on September 
15 without being perfectly matured, and the ears were incompletely de- 
veloped. Some of the observations in regard to the number of internodes, 
length and diameter of stalks, etc., are given in the table following, the 
figures representing the average in each case taken from six typical stalks 
in each row: 
From this table we observe that in each case the stalks developed from 
embryos were shorter than those developed from kernels, and this fact 
is explained by the decrease in the number of internodes which appeared 
in the stalks developed from embryos. Also the diameter of stalks was 
smaller in the plants developed from embryos. While there seemed to 
be no rule governing the node on which the ear appeared, it was noticed, 
