ENDOTHIA PIGMENTS 
345 
approaching crimson magenta. The dry pigment is soluble in dilute 
aqueous solutions of sodium or potassium or ammonium hydroxides, 
or sodium or potassium carbonates. 
Fig. 6. Photomicrograph of crystals of pigment B on the mycelium of E. 
fluens grown on rice. (The writers are indebted to Dr. Erwin F. Smith for this 
photograph.) 
As was mentioned earlier, not all pigments extracted from the 
ground-up rice and mycelium with alcohol were readily soluble in 
ether. The residue remaining after the ether extraction in the case 
of the pigment mixture from E. fluens was considerable. The cultures 
of this fungus on rice, as has been pointed out, show a brilliant purple 
color in the medium while the mycelium on top is yellow. In the 
present study a red pigment frequently crystallized out on the mycelium 
of E. fluens, fig. 6. These crystals were not observed in cultures of 
E. parasitica or E. tropicalis. It was noticed also that when the 
concentrated alcoholic extract was treated with water the water was 
a brilliant red. 
These facts seemed to indicate that part of the pigment A was 
alkaline and soluble in water and slightly soluble in ether, or that 
some other pigment was formed by this species of Endothia along with 
pigment A. The red watery solution obtained from the first precipi- 
tation of the yellow pigment was evaporated to dryness and taken up 
in hot dilute alcohol. The dark red solution thus obtained was con- 
