346 
LON A. HAWKINS AND NEIL E. STEVENS 
centrated in a desiccator and a red crystalline precipitate was formed. 
The residue from the first precipitation of the pigment of E. fluens 
after the extraction with ether was treated with alcohol. The greater 
part of this residue dissolved. When this solution cooled a red crystal- 
line precipitate was formed similar to that obtained from the watery 
extract mentioned above. The crystals were red glistening plates and 
were optically active. After two recrystallizations an acetyl derivative 
was prepared according to the method already described. The com- 
pound crystallized out of absolute alcohol in colorless needles. It was 
recrystallized twice and dried. It melted at 196° to 197° C. uncor- 
rected. A portion of the acetyl derivative was broken down with 
sulphuric acid after the method followed with the acetyl derivative 
of pigment A , and a pigment was obtained which had much the same 
appearance as the original red pigment, which will be designated as 
pigment B in this paper. 
The properties separating pigment B from pigment A are: It 
forms a different acetyl derivative, is only slightly soluble in ether, 
insoluble in toluene, carbon-tetrachloride, petroleum ether, and con- 
centrated nitric acid. It is soluble in water and may be crystallized 
from a water or dilute alcohol solution. When a dilute solution is 
treated with a drop of ferric chloride it becomes darker, assuming a 
greenish raw-sienna color. The alcoholic solution when made slightly 
alkaline closely approaches orange vermillion in color. Crystals of 
the red pigment found on the mycelium of E. fluens were removed and 
tested with various reagents. The reactions were apparently the 
same as those just described. 
Pigment B has not been discovered in cultures of either E. parasitica 
or E. tropicalis grown on starchy culture media. It may be elaborated 
by these two fungi, but if so it occurs in such small amounts as to 
render detection exceedingly difficult. 
It was mentioned in the description of the work with pigment A 
that the yield of acetyl derivative obtained from the yellow precipitate 
from the extract from E. parasitica was very small as compared to 
the yield from a similar amount of the yellow pigment from E. tropicalis 
or E. fluens. It was also shown that the alcoholic solution of the 
pigment from E. parasitica grown on rice has a considerably different 
spectral transmission than that from E. tropicalis. These consider- 
ations made it seem quite possible that another pigment might be 
present in the solution in addition to pigment A. 
