348 
LON A. HAWKINS AND NEIL E. STEVENS 
solvents. They are not in all cases soluble in the same reagent, 
however, and these points of difference are of use in distinguishing and 
separating the pigments. 
Table I 
Solubility of Pigments A, B and C, in Various Reagents at Room Temperature 
Reagent 
Pigment 
A 
B 
C 
Acetic acid 
Soluble 
Soluble 
Soluble 
Acetic ether 
Acetone 
Ethyl alcohol 
Methyl alcohol 
Amyl-alcohol (normal) 
Ether 
Slightly soluble 
Insoluble 
Benzol 
Soluble 
Carbon-tetrachloride 
Insoluble 
Chloroform 
Carbon-bisulphid 
Petroleum ether 
Slightly soluble 
Insoluble 
Sulphuric acid 
Soluble 
Soluble 
Soluble 
Nitric acid (cone.) 
Insoluble 
Water 
Insoluble 
Soluble 
Insoluble 
The statement by Pantanelli that the coloring matter of E. para- 
sitica is a lipochrome seems hardly to be corroborated by the evidence 
brought out in these experiments. Lipochromes according to Zopf^ 
and Samuely^^ break down readily when exposed to light and air. 
They are soluble with a green color in concentrated sulphuric and nitric 
acids. When saponified by boiling with dilute sodium hydroxide 
they are insoluble in alcohol. According to Zopf they are soluble in 
petroleum ether and insoluble in water. 
The pigments obtained in this study did not break down when 
exposed in solution to light and air. Solutions were kept in the 
laboratory for more than a year without apparent deterioration. 
Pigment A was the only pigment which gave a green color when dis- 
solved in concentrated nitric or sulphuric acids. Pigment B was 
insoluble in this last mentioned reagent. Boiling in a dilute solution 
of sodium hydroxide did not, apparently, affect the solubility of the 
pigments in alcohol. Pigment A was the only pigment soluble in cold 
^ Zopf, W. Die Pilze. Page 144. Breslau, 1890. 
Samuely, F. Abderhalden's Handbuch der Biochem. Arbeitsmethoden II: 
758, 1910. 
