376 
A. B. STOUT 
the offspring of A and Ej, four were self-fertile. Seventeen plants, 
the offspring of C and Ej, were self-sterile, as were 30 plants from seed 
of a cross'between a white-flowered plant, {A XC) no. i, of wild stock 
and a plant of the variety "improved striped-leaf." The number of 
self-fertile plants, therefore, varied greatly in the different series, but 
in no series was the proportion very large. 
The self-fertile plants mentioned above appeared after only one 
generation of ancestry known to be self-sterile. Furthermore, the 
parents in each cross were not closely related and were somewhat dif- 
ferent in vegetative habit and flower color. As previously recognized 
(1916, p. 415), these results raised some question regarding the in- 
fluence of wide-crossing as compared with that of inbreeding on the 
development of self-compatibilities, especially as continued inbreeding 
in the variety "red-leaved Treviso" had in two generations given only 
one feebly self-fertile plant out of a total of 49 plants (complete data 
given in 19 16, Table 7). 
In order to obtain further data on this question, it was planned to 
continue inbreeding within this variety, increasing the number of 
plants grown in 19 16, and at the same time to grow for comparison 
an Fi generation from crosses between plants of this variety and a 
self-sterile plant of a wild stock. The present paper will deal especially 
with the data obtained from these cultures. 
Description of the Cultures 
The variety "red-leaved Treviso" is a cultivated salad chicory that 
has been developed in continental Europe. As grown for commercial 
seed production the variety is biennial, seed being sown one summer 
for a crop that matures in the following summer. As grown in my 
culture the plants are more nearly annual. Seed is sown in January 
in flats, and the seedlings are potted and kept in continuous growth 
in the greenhouse until spring, when they are planted in the field. 
Under such treatment the plants, as a rule, reach full development in 
the following August. The general habit of growth of the mature 
plants is well shown in text-figure i. The height has ranged from 4^/2 
to 6^/2 feet with the greater number of plants about 5 feet tall. The 
plants are rather sparsely branched near the base but rather abundantly 
branched above. In the early stages of growth the rosette leaves are 
numerous, of large size, and erect. One of the marked characteristics 
of the family I have grown is the development of a type of fasciation 
