458 
JOHN A. ELLIOTT 
occasionally produced around needle pricks but no spores were pro- 
duced and the parasitism was evidently very feeble. 
M. sarcinula from apple fruit spot: This fungus was isolated by 
Miss Jean Maclnnes, of the University of Illinois, in her studies of the 
rots of apples.^ It appeared to be causing an apple rot. Small black 
pycnidia-like bodies were scattered frequently over the rotted area 
under the epidermis of the apple. These also appeared in the first 
generation on agar. When broken they appeared to be merely 
sclerotia. Spores from pure cultures were used in making inoculations 
both by needle pricks and on uninjured leaf surface. The fungus 
conformed to descriptions and figures of M. sarcinula. The same 
plants were inoculated with M. sarcinula as with M. sarcinaeforme. 
Spots were produced only on red clover and alfalfa. All attempts to 
inoculate apples failed. The spots on red clover were lighter colored 
but otherwise almost identical with those caused by M. sarciniae- 
forme. Few spores were produced on the spots. On the onion tops 
the fungus produced small white spots surrounding the punctures but 
no spores were formed. 
The most noteworthy results of the inoculation experiments oc- 
curred in respect to A. solani, A. sonchi, A. brassicae var. nigrescens, 
and M. sarcinula. Both strains of A. solani grew freely on all of the 
solanaceous hosts, and one strain was feebly parasitic on cabbage. 
Morphologically, judging from exsiccati, A. solani and A. brassicae 
are identical, and the inoculation experiments might be considered 
further evidence of this view. A. sonchi was actively parasitic on 
Lactuca, a near relative of Sonchus, and was possibly very feebly 
parasitic on other Compositae. A. brassicae var. nigrescens proved 
slightly parasitic on cabbage although this would hardly be expected 
from nearness of Cruciferae and Cucurbitaceae. M. sarcinaeforme 
and M. sarcinula are entirely dissimilar except in general form of 
spores, yet they appeared equally parasitic on red clover and alfalfa, 
and both were feebly parasitic on onion tops. 
Cultures on Agar 
For purposes of isolation and general study, standard lima bean 
agar (beans lOO g., agar 15 g., water 1,000 g.) was used. The cultures 
were kept in darkness at 30° C, with the exception that A. sonchi, 
which would not grow at 30°, was grown at 20°. In testing the effect 
^ Unpublished thesis. 
