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ferring to this particular vaccine during the following experi- 
ments it will be called the vaccine of June 1 to distinguish it 
from other vaccines that may have been used elsewhere prior 
to that date. This particular vaccine gave such excellent re- 
sults — in fact, beyond expectation — that it deserves special 
mention. 
Experiment No. 1 — Breed, Single Comb White Leghorns; 
225 pullets, age three months. The disease was first noted on 
June 10, and in going over the flock it was observed that the 
infection was just commencing to show itself. About 20% 
of the pullets showed lesions on the upper and lower lids of 
the eye and some on the beak. The scabs were not removed ; 
nor were they treated at all. One mil. of vaccine was injected 
on the left side of the bird near the keel of the sternum or 
that part of the breast bone near the crop. The skin is loose 
there and easily picked up for a subcutaneous injection. All birds 
were treated. 
The usual second treatment, five days later, was omitted. 
The surprising part of this experiment was that with the one 
injection of vaccine the disease was checked and the infected 
birds made a complete and rapid recovery. Mortality none. 
This rapid recovery, without doubt, was due to the vaccine. 
Credit also must be given to the physical condition of the 
flock at that time as they were strong. 
Experiment No. 2 — Breed, Single Comb White Leghorns ; 85 
pullets, 55 cockerels, age 8 weeks. These chicks were treated 
as they were taken from the crates in being transferred from 
the brooder house to colony houses. Infection was light, only 
seven cockerels showing sorehead. Both lots of birds were 
inoculated with the vaccine of June 1, giving only one treat- 
ment, the dosage being the maximum 1 mil. As one can 
imagine, the results were closely watched. The disease was 
checked and recovery in the seven cockerels was rapid. Mor- 
tality none. 
Experiment No. 3 — Breed, Single Comb White Leghorns ; 
90 chicks, 6 weeks of age. Two pens, 45 to the pen. Only 
one bird showed infection and both pens were treated with 
this vaccine of June 1, using but one-half mil. One injection 
was given and the results excellent. Morfality none. 
On September 4, 39 eight-weeks' old pullets and three eight- 
weeks' old cockerels and two two-months' old turkeys were 
treated. The pullets were free from any visible signs of in- 
fection, but the cockerels and turkeys showed fair size lesions 
on the combs and head. One mil. of voccine of Tune 1 
was given and not repeated. The results were a repetition of 
the three previous experiments. Mortality none. 
Special attention must be called to the fact that this vaccine 
of June 1 was over 60 days old when used on this last experi- 
ment. With vaccine of this quality, used with ordinary intel- 
ligence, anyone raising poultry in Hawaii should be able to 
