A GLOEOSPORIUM DISEASE OF THE SPICE BUSH 34I 
readily develop within twenty-four hours when placed in a moist 
chamber. A pure culture of the Gloeosporium may be readily obtained 
from both infected leaves and stems of the spice bush by first sterilizing 
the surface, then placing bits of tissue in agar plates. 
Pathogenicity. — The method of inoculation was to spray the leaves 
and fruits of the spice bush with an atomizer containing a heavy 
suspension of the Gloeosporium spores from a pure culture in sterile 
water. The inoculated parts were then enclosed in lamp chimneys 
and both open ends closed with sterile non-absorbent cotton. Checks 
were treated similarly but they were sprayed with distilled water. 
Ten chimneys were used at one time, two for the checks and eight for 
the inoculations. The experiment was repeated three times, and the 
lamp chimeys were not taken off before twenty-four hours. After 
each experiment the lamp chimneys were immersed in 5 per cent, 
formaldehyde for ten minutes, then rinsed in sterile water. Typical 
infections began to appear on the tender leaves in from three to eight 
days, but the older leaves remained healthy. The inoculated fruits 
also showed the typical spotting. All the checks remained healthy. 
The symptoms obtained from the artificial inoculations were the same 
and identical with those of the natural infections. The above ex- 
periments were carried on in June and July of 1909. In the fall of 
that same year young spice bush seedlings were dug out in the woods 
and planted in sterile pots and soil in the laboratory. The seedlings 
were kept for four weeks. Two dried up and were discarded, eight 
made a good start and showed no disease. Two of these plants were 
left as checks and six sprayed with a heavy suspension of the Gloeo- 
sporium spores from a pure culture originally isolated from the diseased 
spice bush fruit. All the eight plants were covered with bell jars 
for twenty-four hours. A week after infection the tip leaves of the 
inoculated seedlings turned brown and died, whereas the leaves on the 
check plants remained healthy. The fungus was readily re-isolated 
from the artificially infected leaves, and these when placed in a moist 
chamber for twenty-four hours were covered with a layer of acervuli 
of salmon-colored spore masses. 
Identity of the spice hush Gloeosporium. — I have already mentioned 
that Gloeosporium officinale E. & E. has been reported on sassafras 
leaves. In pure cultures this fungus and the spice bush Gloeosporium 
cannot be distinguished from Gloeosporium fructigenum Berk, of the 
apple. Typical infections on the spice bush were obtained with spores 
