THE LAMELLAE IN COPRINUS MICACEUS 
347 
make clear what becomes of the upper portion of the young carpophore 
in the annulate agarics which is present before the development of the 
hymenium when the so-called pileus primordium is formed. 
In studying the nuclear phenomena in the mycelia of a number 
of pileate fungi I found that Coprinus micaceus grows well in culture 
media and is favorable material for studying the formation of the 
various structures of the carpophore, particularly the lamellae. 
Methods 
The carpophores of Coprinus micaceus were collected in great 
numbers during the summer of 191 3 in the vicinity of New York. 
Petri dishes thoroughly cleaned and partly filled with water were 
covered with circular pieces of oak tag in which two to three circular 
openings were made somewhat smaller than the diameters of the pilei. 
The caps were placed over these openings and the Petri dishes were 
covered. After six to ten hours when the spores had been sown the 
caps were removed and the pieces of oak tag covered with spores were 
likewise taken from the dishes and carefully folded and preserved. In 
tap water germination occurs in about twenty-four hours. The 
germinating spores were transferred to pint milk bottles containing 
various media (a) soil in which the plants had been growing; {h) soil 
mixed with horse dung; (c) horse dung; {d) pieces of wood taken from 
the same soil; {e) beef agar; (/) carrot; {g) beans; {h) beet leaves; {i) 
string beans; (7) horse dung agar; {k) corn meal agar. From forty to 
sixty days after inoculation young carpophores appeared on the soil, 
dung and cornmeal agar media. In cases where the medium 
became too dry sterilized water was carefully added by means of a 
pipette. 
Buttons were carefully removed from the cultures and fixed. The 
substratum was teased away and the young carpophores were trans- 
ferred to vials. In case of the agar media the agar was cut away and 
buttons and medium together were put in a fixing bath. A number 
of fixing solutions were used, Bouin's, Merkel's, picro-acetic, and 
chrom-acetic. The best results were obtained, however, with Flem- 
ming's weaker mixture. After twelve to twenty-four hours the material 
was washed in running water, carefully dehydrated and imbedded in 
52° parafiin. Sections 5 m to 7.5 )U in thickness were made. The 
preparations were stained with iron haematoxylin, eosin, acid fuchsin, 
and Congo red, but principally with Flemming's triple stain. 
