EXPULSION OF ASCOSPORES 
but one specimen had expelled ascospores. The expulsion was on 
the whole rather moderate. The control kept at laboratory tempera- 
ture, about 72° F., shot abundantly. 
(b) Temperature ^2,8°-^4.()° F. Average 5J.5° F. The specimens 
were collected at Oxford, Chester Co., Pa., Dec. 30, 1912, prepared 
as described above and the experiment started Jan. 6, 1913 with 20 
specimens for the experiment proper and 19 for the control. The 
trays were kept in a cold room for two weeks at an average tempera- 
ture of 53.8° F. Shooting was very light, as may be noted from the 
following summary: 
Table I 
Number of specimens showing expulsion at 53.8° F. 
No. of days shooting occurred o i 2 3 4 
No. of specimens 6 5 4 2 3 
It may be seen from this tabulation that 6 specimens showed no 
expulsion whatever for the entire period of two weeks, while the 
maximum amount was given by three specimens which gave expulsion 
on four different days. After the first two days only single spots were 
obtained from any specimens. At the end of two weeks they were 
moved to laboratory temperature, about 72° F. for 12 days where 
expulsion occurred from all but one specimen. Shooting was moder- 
ate. The trays were again moved to the cold room, this time for 13 
days, at an average temperature of 54.9° F. For the first two days 
expulsion continued as at laboratory temperature but after this time 
it was very light and corresponded to the first two weeks in the cold 
room. The control at the laboratory temperature, average 72° F., 
shot abundantly. 
(c) Temperature S4°~59° Average 56.2$° F. The specimens 
were collected at Emilie, Bucks Co., Pa., Nov. 19, 1913, and prepared 
according to the regular methods, but the experiment was not started 
until Dec. 3, 1913. This delay was caused for the reason that the 
cold room had not been adjusted to the proper temperature. The 
trays for the experiment were put in the cold room and those for the 
control in the laboratory Nov. 20, 1913 and the blotting paper in the 
trays moistened each day to prevent the specimens from drying out. 
Dec. 3, 1 91 3, the bark was wet and the test started with 12 specimens 
for the experiment and 12 for the control. The trays were kept in 
the cold room for 10 days at an average temperature of 56.25° F. 
