EXPULSION OF ASCOSPORES 
was used to furnish the required temperature. 12 specimens were 
used for the experiment proper and 12 for the control. Two trays of 
specimens were put in the incubator on Nov. 6, 1913, 3 hours previous 
to wetting the bark and starting the experiment. This was done to 
equalize the temperature of all. A thermograph and a beaker of 
water for use in moistening the specimens were also kept in the 
incubator. The test was started at 3:00 P.M., on November 6 and 
continued for 9 days at an average temperature of 79° F. At this 
temperature the specimens seemed to shoot abundantly and just as 
actively as the control specimens. There was but little appreciable 
difference either in density or number of the spots; although the 
number of active ostioles per trap per day for all controls was 72.6, 
and 84.5 for the specimens at 79° F. (see Table II). 
(g) Temperature 82°-86.^° F. Average 84.6° F. Specimens were 
collected at Emilie, Pa., Nov. 19, 1913, the customary methods used 
in their preparation, and the experiment started November 20. 
The same incubator was used as for the preceding (79°) experiment. 
The trays of specimens were put in the incubator at 3:30 P. M. in 
order to equalize the temperature but the bark was not drenched 
with water or slides adjusted until 10:00 A. M. The test was con- 
tinued for II days, during which time all of the 12 specimens showed 
active ostioles but expulsion was rather moderate and could not be 
termed abundant except in some few individual cases on certain days. 
The control specimens shot abundantly. For the first eight days, 
while the amount of expulsion from the control specimens exceeded 
that from test specimens, it was more noticeable during the last three 
days when the effect of the high temperature began to be very evident. 
After the first day the density of the spots was always greatly in favor 
/ of the control. On the whole the amount of expulsion at 84.6° F. 
should be characterized as medium in comparison with the abundant 
expulsion at the more favorable temperature (see Table II). 
(h) Temperature 8q°-Q2.^° F. Average qo.8° F. Specimens were 
collected Oct. 23, 191 3, at Emilie, Pa., and prepared the same as the 
preceding ones. 12 specimens were used for the experiment proper 
and 12 for the control. An incubator was used as in the two preceding 
tests, to obtain the desired temperature. The specimens were pre- 
pared on October 24 and kept moist until October 27 by wetting only 
the blotting paper. Absorption from the paper however furnished 
sufficient moisture in 17 out of the 24 specimens to cause considerable 
