EXPULSION OF ASCOSPORES 5 15 
only^a few "comet's tails" are formed and this is probably when 
the slide is of medium distance from the ostiole. Unless the slide is 
'very close spores from obliquely directed necks never reach the slide. 
Fig. 2. Spore print of ascospores of Endothia parasitica obtained from perithecia 
in an inverted position. The comet-like spots are very characteristic. 
The Effect of Saturated Atmosphere on Ascospore Expulsion 
In order to determine the effect of a saturated atmosphere upon 
ascospore expulsion, two tests were run at laboratory temperature. 
The specimens used were collected at Emilie, Pa. The experiments 
are as follows: 
Three specimens of excellent perithecial material were selected for 
the test and two of them put in a large closed damp chamber on wet 
blotting paper while the third was kept as a check in an open chamber. 
The specimens were wet, slides adjusted and the experiment started 
Jan. II, 1913. Records were taken for 27 days at the end of which 
time the control was found to have shot 7 1 spots to an average of 1 33 
for the other two. 
