INFLUENCE OF ILLUMINATING GAS ON BACTERIA AND FUNGI 
5 
number of others, Bacillus carotovorus Jones, B. melonis Giddings, 
B. campestris Pammel, B. mycoides Fliigge, B. solanisaprus Harrison, 
Pseudomonas radicicola (Bey.) Moore, Bacterium stewarti Erw. Smith 
and B. tumefaciens Erw. Smith, were secured from the American 
Museum of Natural History through the botanical department of the 
University of Michigan. The following fungi were used: Oidium 
lactis Fresenius, obtained from the department of bacteriology, a 
strain of Penicillium stoloniferum Thom, isolated from moldy bread 
and determined by Miss Margaret B. Church, of the U. S. Depart- 
ment of Agriculture, a previously undescribed yeast, ^ isolated from 
the air at the University of Michigan, Penicillium pinophilum Hedg- 
cock, P. camemherti Thom, P. roqueforti Thom and P. expansum Link, 
the last four of which were obtained from Miss Margaret B. Church 
through the courtesy of Dr. Charles Thom, and the four species, Fusa- 
rium radicicola Wollenw., Gleosporium cingulata- Atkinson, Endothia 
parasitica (Murr.) P. J. & H. W. And. and E. fluens (Sow.) S. & S., 
which were received from Dr. Lon A. Hawkins. 
The cultures were carried on ordinary i percent glucose, i percent 
peptone, 0.3 percent beef extract agar, with 0.5 percent sodium chloride 
and 1.5 percent agar, except that some of the experiments with B. 
rubidus were carried out on autoclaved potato slants. The color 
which B. rubidus develops on this substratum made the medium of 
^ I take this opportunity of thanking Dr. H. W. Anderson for help with this 
species. He has made a taxonomic study of it and has kindly furnished the following 
diagnosis: 
Cryptococcus Ludwigi H. W. Anderson sp. nov. 
Morphology. — Cells round or oval, becoming elliptical in old cultures. Cyto- 
plasm very coarsely granular. A single large granule usually evident. Buds arising 
from any point but usually from shoulders in elliptical cells. Size 3.5 X 4.5 
Cultural characters. — On dextrose agar the streak is filiform, at first light pink, 
slimy, smooth, later becoming dry and very decidedly wrinkled and heaped. The 
dry, wrinkled type of growth is peculiar to this species of pink yeasts. On carrot 
and other solid media the streak has the same type of growth. In gelatin stab the 
line of puncture is filiform with no liquefaction. 
Biochemical properties. — There is no fermentation of dextrose, lactose, galactose, 
sucrose, levulose nor rafifinose. Litmus milk is rendered more alkaline. 
From culture No. 51. Type specimen No. 51. Type slide No. 51. Cultures 
have been sent to several laboratories; and type slide, culture and dried material 
have been deposited in the herbarium of the University of Illinois. The organism 
was isolated from the air at the botanical laboratories of the University of Michigan, 
Ann Arbor, Michigan. 
