64 
HALLY JOLIVETTE SAX 
one large nucleus with a distinct nucleole. There is present a clear 
staining central body, which in this case is opposite the nucleole. 
The chromatin is distributed in a fine network and shows more or less 
orientation with regard to the central body, i. e., the chromatin 
granules run inward from the center in more or less distinct rows. 
The cytoplasm is comparatively dense at this stage. This stage is 
only of short duration as the ascus elongates rapidly, taking the more 
slender form seen in figure 3. 
Figure 3 represents a young ascus, which is very slender and 
cylindrical in shape. At this time the nucleus is usually situated in 
the central portion of the ascus, in this case being nearer the lower 
end. The cytoplasm is rather uniformly distributed and appears to 
have a fine granular structure. A few radiations in the cytoplasm 
extend outward from the nucleus. The nucleole is not visible in this 
section. The chromatin is in fairly large masses for the most part, 
and some connection is visible between those particles. 
The ascus undergoes a rapid increase in size, so that when the first 
division of the nucleus occurs it appears to have become several times 
its original size. The first division of the primary nucleus is shown 
in figure 4. The cytoplasmic mass is very great compared with that 
of the nucleus. The cytoplasm in the upper part of the ascus is very 
similar to that shown in figure 3. The cytoplasm of the lower portion 
appears a little more vacuolate. The spindle figure is somewhat 
curved and very distinct and has a small but clearly staining central 
body at each end. The chromosomes are few in number. They are 
short and stain heavily. Fibers extend out from the centers but 
they are not at all distinct between the plasma membrane of the 
ascus and the centers in this figure. However, there is a very notice- 
able bulg^ing out of the membrane opposite the central bodies which 
may indicate some relation between the membrane and the division 
figure, such as the radial arrangement of astral rays extending outward 
from the central bodies. Fibers may be seen extending upward and 
downward from the central bodies. Those below the spindle were 
especially distinct and in the plane at which figure 4 was drawn they 
appear to converge at a considerable distance from the central bodies, 
making the astral rays appear considerably longer than those of the 
spindle. The division figure lies crosswise of the ascus. 
The nuclear divisions in the ascus follow one another rapidly. 
The daughter nuclei divide while still in close proximity to each other. 
