SPORE FORMATION IN PHILOCOPRA COERULEOTECTA 65 
Thus the nuclei occupy the part of the ascus nearest the place where 
the division of the primary nucleus occurred. The daughter nuclei 
in each case are completely formed before entering upon a new divi- 
sion. The spindle is intranuclear at first. Often the nuclear cavity 
remains distinct until the early anaphase. 
Figure 5 shows two of the resting nuclei in the four-nucleated stage 
of the ascus. In this case the ascus was cut obliquely. The nuclei 
are fairly large and show much the same structure as was shown in 
the primary nucleus of the ascus. The nucleole and central body 
are distinct. The chromatin network is typical of that formed in 
the few-spored Ascomycetes and shows some orientation with respect 
to the central body. It has a lighter and a darker staining portion, 
the darker part forming lumps scattered along the finer light-staining 
thread-like portion. The cytoplasm is somewhat denser near the 
nuclei but there is no well-marked spore-plasm. 
Figure 6 represents a dividing nucleus of the third division. One 
complete figure is shown which runs almost parallel to the length of 
the ascus. The spindle is slender. The chromosomes have divided 
and are passing toward their respective poles, where there is a small 
distinctly staining central body. The nuclear cavity is still apparent. 
There is here an interesting arrangement of the cytoplasm with regard 
to the central bodies. It is very dense in the immediate neighborhood 
of the centers, from which distinct radiations extend outward in all 
directions. To the right of the lower central body there is visible 
an oblique section through the polar end of a second spindle figure. 
The polar aster is apparent. The cytoplasm is fine with radiations 
running outward in all directions. They are very fine and dense in 
the immediate neighborhood of the central body. 
In the eight-nucleate stage (fig. 7) the ascus has grown somewhat 
larger. The nuclei are close together in the region near where the 
primary nucleus was located. The cytoplasm is denser immediately 
surrounding the nuclei and more vacuolate in that further remote. 
The less uniform appearance of the cytoplasm is conspicuous in com- 
parison with the earlier stages. The nuclei appear to be in a normal 
resting condition. They are much smaller than the primary nucleus. 
Each nucleus contains a distinct nucleolus. The chromatin appears 
in fine granules. The central body is visible in the best stained speci- 
mens, but is not always apparent. Only three of the eight nuclei are 
visible in the section. 
